Wagner P, Fuchs A, Prowald A, Montenarh M, Nastainczyk W
University of Saarland, Homburg/Saar, Germany.
FEBS Lett. 1995 Dec 18;377(2):155-8. doi: 10.1016/0014-5793(95)01329-6.
Originally identified as multicopy suppressor of a lethal growth arrest caused by expression of a tumour mutant cDNA of p53 in fission yeast the tms1 gene product was found to form stable complexes with p53 in yeast. By using purified recombinant proteins multimeric complexes of tms1 and p53 could be demonstrated and recently the p53 binding site on the tms1 protein was established to the sequence YYITTEDFCT (aa 116-125) in the vicinity of a well conserved cell division motif. Here we report the precise mapping of the tms1 binding site on the p53 protein to the sequence LQIRGRERFE (aa 330-339) which defines a new functional domain on the p53 protein.
最初在裂殖酵母中，tms1基因产物被鉴定为p53肿瘤突变cDNA表达所导致的致死性生长停滞的多拷贝抑制因子，发现它能在酵母中与p53形成稳定复合物。通过使用纯化的重组蛋白，可证明tms1和p53的多聚体复合物，最近确定tms1蛋白上的p53结合位点为保守细胞分裂基序附近的YYITTEDFCT序列（氨基酸116 - 125）。在此，我们报告tms1蛋白在p53上的结合位点精确映射至LQIRGRERFE序列（氨基酸330 - 339），该序列定义了p53蛋白上一个新的功能结构域。
