Wagner P, Fuchs A, Nastainczyk W
Int J Oncol. 1995 Jul;7(1):171-5.
Recently we described the cloning of the yeast tms1 gene by complementation of a lethal growth arrest caused by expression of a tumour mutant cDNA of p53 in fission yeast. The tms1 gene product was found to form stable complexes with p53 in yeast and in vitro using purified recombinant proteins the interaction was mapped to the C-terminal region of p53. Using a combination of a genetic and a synthetic approach we were able to establish the p53 binding site on the tms1 protein to the sequence YMITTED FCT (aa 116-125) in the vicinity of a well conserved cell division motif.
最近，我们描述了通过补充裂殖酵母中由p53肿瘤突变体cDNA表达引起的致死性生长停滞来克隆酵母tms1基因的过程。发现tms1基因产物在酵母中与p53形成稳定的复合物，并且在体外使用纯化的重组蛋白将这种相互作用定位到p53的C末端区域。通过遗传和合成方法的结合，我们能够确定tms1蛋白上的p53结合位点为保守细胞分裂基序附近的序列YMITTED FCT（第116-125位氨基酸）。
