UNIV SAARLAND,DEPT MED BIOCHEM,D-66421 HOMBURG,GERMANY. KAROLINSKA INST,DEPT CHEM 1,S-17177 STOCKHOLM,SWEDEN.
Int J Oncol. 1994 Sep;5(3):667-73. doi: 10.3892/ijo.5.3.667.
Expression of the growth suppressor gene p53 in Schizosaccharomyces pombe causes growth arrest which can be suppressed by overexpression of a yeast gene termed tms1. The tms1 gene encodes a 37 KDa protein which binds to p53 in vitro and in vivo when p53 was expressed in fission yeast cells. By using antibodies against recombinant tms1 protein we analyzed mammalian cells for the expression of a tms1 related protein. Human, monkey and rat cells exhibited a bright nuclear fluorescence when incubated with the tms1 specific antibody. In addition, we were able to specifically immunoprecipitate a 42 KDa protein which forms complexes with the p53 protein. The specificity of these complexes was demonstrated by coimmunoprecipitation using different monoclonal antibodies against p53, antibodies against yeast tms1 as well as by Western blot analysis. Thus, we found that the mammalian tms1 related protein represents a new p53 binding protein.
p53 生长抑制基因在裂殖酵母中的表达会导致生长停滞，而酵母 tms1 基因的过表达可以抑制这种停滞。tms1 基因编码一个 37kDa 的蛋白质，当 p53 在裂殖酵母细胞中表达时，该蛋白质可以在体外和体内与 p53 结合。我们利用针对重组 tms1 蛋白的抗体，分析了哺乳动物细胞中 tms1 相关蛋白的表达情况。用 tms1 特异性抗体孵育人、猴和鼠细胞时，细胞核呈现明亮的荧光。此外，我们还能够特异性地免疫沉淀与 p53 蛋白形成复合物的 42kDa 蛋白。这些复合物的特异性通过使用针对 p53 的不同单克隆抗体、针对酵母 tms1 的抗体以及 Western blot 分析进行了验证。因此，我们发现哺乳动物的 tms1 相关蛋白是一种新的 p53 结合蛋白。
