Nagel G, Möckel B, Büldt G, Bamberg E
Max-Planck-Institut für Biophysik, Frankfurt, Germany.
FEBS Lett. 1995 Dec 18;377(2):263-6. doi: 10.1016/0014-5793(95)01356-3.
We report on the first successful expression of the light driven H+ pump, bacteriorhodopsin, into the plasma membrane of oocytes from Xenopus laevis. The light induced photocurrents which reflect the pumping of H+ by BR were analysed under voltage clamp conditions. At least 100 active BR molecules per microns 2 were expressed in the plasma membrane so that both the voltage clamp and giant patch clamp method could be applied. We show that H+ pumping by BR is modulated by the membrane potential, i.e. the pump current shows strong voltage dependence in the range measured between -165 mV to +60 mV.
我们报道了光驱动的H⁺泵——细菌视紫红质首次成功表达于非洲爪蟾卵母细胞的质膜中。在电压钳制条件下，分析了反映细菌视紫红质泵出H⁺的光诱导光电流。质膜中每平方微米至少表达了100个活性细菌视紫红质分子，因此电压钳制和巨膜片钳制方法均可应用。我们发现细菌视紫红质的H⁺泵浦受膜电位调节，即在-165 mV至+60 mV测量范围内，泵电流表现出强烈的电压依赖性。
