Sverdlova A N, Nefelova M V, Silaev A B
Antibiotiki. 1979 Jan;24(1):7-11.
An orange antibiotically active substance isolated from the mycelium of a mutant strain of Actinomyces chrysomallus var. carotenoides was identified as a mixture of actinomycins according to its light absorption spectra, circular dichroism spectra, IR spectra and chromatographic comparison with the standard samples. A scheme for successive extraction of the biologically active substances from the mycelium resulting in isolation of a fraction enriched with antibiotic substances and a fraction enriched with pigments is presented. A method for separation and purification of 3 groups of biologically active substances from the mycelium enriched extract was developed.
从金黄色放线菌胡萝卜素变种突变株的菌丝体中分离出的一种橙色抗生素活性物质，根据其光吸收光谱、圆二色光谱、红外光谱以及与标准样品的色谱比较，被鉴定为放线菌素混合物。本文提出了一种从菌丝体中连续提取生物活性物质的方案，该方案可分离出富含抗生素物质的馏分和富含色素的馏分。还开发了一种从富含菌丝体的提取物中分离和纯化3组生物活性物质的方法。
