Ittmann M M, Wieczorek R
Department of Pathology, New York Department of Veterans Affairs Medical Center, NY 10010, USA.
Hum Pathol. 1996 Jan;27(1):28-34. doi: 10.1016/s0046-8177(96)90134-3.
Alterations of the retinoblastoma (Rb) tumor suppressor gene and its encoded protein have been detected in a variety of malignant neoplasms. The authors have evaluated a series of 26 clinically localized stage B prostate adenocarcinomas for loss of heterozygosity (LOH) at 13q14 (including the Rb locus), rearrangements or partial deletions of the Rb gene, and alterations of Rb protein level by quantitative immunohistochemistry. LOH at the Rb locus occurred in 35% of the informative specimens. Of the specimens that showed LOH, 33% also had decreased or absent Rb protein in tumor cells by quantitative immunohistochemistry. In contrast, none of the specimens without LOH showed loss of Rb protein. Thus, LOH is correlated with loss of Rb protein. The authors conclude that alterations of the Rb tumor suppressor gene occur in a significant fraction of stage B prostate adenocarcinomas.
视网膜母细胞瘤（Rb）抑癌基因及其编码蛋白的改变已在多种恶性肿瘤中被检测到。作者评估了一系列26例临床局限性B期前列腺腺癌，检测其13q14（包括Rb基因座）的杂合性缺失（LOH）、Rb基因的重排或部分缺失，以及通过定量免疫组织化学检测Rb蛋白水平的改变。Rb基因座的LOH发生在35%的信息性标本中。在显示LOH的标本中，33%的肿瘤细胞通过定量免疫组织化学检测也出现Rb蛋白减少或缺失。相比之下，没有LOH的标本均未显示Rb蛋白缺失。因此，LOH与Rb蛋白缺失相关。作者得出结论，Rb抑癌基因的改变在相当一部分B期前列腺腺癌中发生。
