Banerjee P, Chakrabarty A N, Dastidar S G
Department of Medical Microbiology, Calcutta University College of Medicine.
Indian J Med Res. 1995 Sep;102:104-13.
The splenic tissue of a mouse experimentally infected with M. lepraemurium (Hawaiian strain, M-65) and developing 'rat leprosy', yielded a pure culture of an acid - fast bacterium having all the characteristics of M. lepraemurium on mineral salt minimal medium supplemented with simple sources of C and N, e.g., NH4 -salts, liquid paraffin, urea, gelatin etc. This could be maintained, by serial passages in vitro with good growth. Its indefinite propagation with tissue - free washed, small inoculum on complex media including Ogawa medium was difficult, and its serial sub-culture was practically impossible. The in vitro isolate from supplemented minimal medium could produce pathological lesions in mice typical of rat leprosy.
用实验方法感染鼠麻风杆菌（夏威夷菌株，M - 65）并患上“大鼠麻风病”的小鼠脾脏组织，在添加了简单碳源和氮源（如铵盐、液体石蜡、尿素、明胶等）的无机盐基本培养基上，产生了一种具有鼠麻风杆菌所有特征的抗酸菌纯培养物。通过在体外连续传代，该培养物生长良好。但使用无组织的洗涤过的小接种量在包括小川培养基在内的复杂培养基上进行无限繁殖很困难，实际上也无法进行连续传代培养。从添加了成分的基本培养基中分离出的体外培养物能在小鼠身上产生典型的大鼠麻风病病理损伤。
