Ishaque M
Can J Microbiol. 1981 Aug;27(8):788-94. doi: 10.1139/m81-122.
The primary in vitro cultures from lepromata of mice or rats previously infected with the Hawaiian strain of Mycobacterium lepraemurium were obtained on Ogawa egg-yolk medium at 34 degrees C in approximately 90 days of incubation. Optimal growth of subcultures was achieved in 40 to 60 days of incubation and such cultures were used to test their pathogenicity in animals. The in vitro grown subcultures provoked in mice subcutaneous lepromata identical to those produced by the in vitro grown M. lepraemurium. Also, mice infected subcutaneously and intravenously with the in vitro grown subcultures developed lesions in livers, spleens, and kidneys similar to those of mice infected with the mouse passage murine leprosy bacilli. Microscopically and histopathologically, the acid-fast bacilli derived from organs infected with the in vitro or in vivo grown cultures were indistinguishable from each other.
将先前感染了夏威夷株鼠麻风杆菌的小鼠或大鼠的瘤型麻风结节进行初次体外培养，在小川蛋黄培养基上于34℃培养约90天获得。传代培养物在培养40至60天时生长最佳，并用这些培养物来测试它们在动物体内的致病性。体外培养的传代培养物在小鼠体内引发的皮下麻风结节与体外培养的鼠麻风杆菌所产生的结节相同。此外，皮下和静脉注射体外培养传代培养物的小鼠，其肝脏、脾脏和肾脏出现的病变与感染小鼠传代鼠麻风杆菌的小鼠相似。在显微镜和组织病理学上，来自感染了体外或体内培养物的器官的抗酸杆菌彼此无法区分。
