Dogra S, Donaldson K
Industrial Toxicology Research Centre, Mahatma Gandhi Marg, Lucknow, India.
Ind Health. 1995;33(3):131-41. doi: 10.2486/indhealth.33.131.
The influence of long and short fibre amosite on the generation of tumor necrosis factor (TNF) by rat alveolar macrophages was investigated in vitro. TNF rich supernatants were prepared from macrophages cultured in F10 medium +2% Bovine Serum Albumin (BSA). Spontaneously released TNF from unstimulated macrophages and TNF rich supernatants from macrophages exposed to Lipopolysaccharide (LPS) and fibres were stored at -70 degrees C and then tested for their cytotoxicity towards L929 cells. Maximum spontaneously released TNF was obtained from 24 hour macrophage cultures. Short amosite fibres had no significant effect in stimulating alveolar macrophages to release TNF while the 50 micrograms dose of long fibres resulted in significantly increased release of TNF. Cotreatment of alveolar macrophages with LPS and fibres further enhanced the TNF production and maximum production was obtained with LPS +50 micrograms dose of long fibre resulted in significantly increased release of TNF. Co-treatment of alveolar macrophages with LPS and fibres further enhanced the TNF production and maximum production was obtained with LPS +50 micrograms of long fibre amosite. The present study indicates that fibre dimension is a major factor in in vitro dust activity and TNF has a possible active role to play in dust induced inflammation in vivo.
体外研究了长短纤维状铁石棉对大鼠肺泡巨噬细胞产生肿瘤坏死因子（TNF）的影响。富含TNF的上清液由在F10培养基+2%牛血清白蛋白（BSA）中培养的巨噬细胞制备。未刺激的巨噬细胞自发释放的TNF以及暴露于脂多糖（LPS）和纤维的巨噬细胞的富含TNF的上清液保存在-70℃，然后检测它们对L929细胞的细胞毒性。24小时巨噬细胞培养物可获得最大自发释放的TNF。短纤维状铁石棉对刺激肺泡巨噬细胞释放TNF无显著影响，而50微克剂量的长纤维导致TNF释放显著增加。肺泡巨噬细胞与LPS和纤维共同处理进一步增强了TNF的产生，LPS +50微克剂量的长纤维导致TNF释放显著增加，LPS与纤维共同处理肺泡巨噬细胞进一步增强了TNF的产生，LPS +50微克长纤维状铁石棉可获得最大产量。本研究表明，纤维尺寸是体外粉尘活性的主要因素，TNF在体内粉尘诱导的炎症中可能发挥积极作用。
