Vaarala O, Saukkonen T, Savilahti E, Klemola T, Akerblom H K
Children's Hospital, Department of Pediatrics, University of Helsinki, Finland.
J Allergy Clin Immunol. 1995 Dec;96(6 Pt 1):917-23. doi: 10.1016/s0091-6749(95)70229-6.
Development of humoral and cellular immune responses to orally administered antigens in human beings is poorly understood, although antigen administration has been suggested as a treatment for hypersensitivity disorders and autoimmune diseases.
The purpose of the study was to investigate the development of systemic immune response in infants fed with formula containing whole cow's milk proteins or hydrolyzed formula containing casein peptides.
In a double-blind trial, 10 infants received cow's milk-based formula, and 10 infants received a casein hydrolysate formula until the age of 9 months. Blood samples were taken at the ages of 6, 9, and 12 months. Cellular responses were assessed by proliferation assay of peripheral blood mononuclear cells to cow's milk proteins (beta-lactoglobulin, bovine serum albumin, and alpha-casein). Humoral responses to the same proteins were measured by ELISA for IgG antibodies.
Feeding infants with cow's milk-based formula induced systemic humoral and cellular responses to cow's milk proteins. T-cell response later declined, supporting the concept of oral tolerization. Exposure to cow's milk proteins after the age of 9 months resulted in depressed cellular and humoral responsiveness to these proteins.
Our results support the view that induction of oral tolerance in human beings is an age-dependent phenomenon.
尽管抗原给药已被提议用于治疗超敏反应性疾病和自身免疫性疾病，但人们对人类口服抗原后体液免疫和细胞免疫反应的发展了解甚少。
本研究旨在调查喂食含全脂牛奶蛋白配方奶粉或含酪蛋白肽水解配方奶粉的婴儿全身免疫反应的发展情况。
在一项双盲试验中，10名婴儿接受基于牛奶的配方奶粉，10名婴儿接受酪蛋白水解配方奶粉，直至9个月龄。在6、9和12个月龄时采集血样。通过外周血单核细胞对牛奶蛋白（β-乳球蛋白、牛血清白蛋白和α-酪蛋白）的增殖试验评估细胞反应。通过ELISA检测针对相同蛋白质的IgG抗体来测量体液反应。
用基于牛奶的配方奶粉喂养婴儿可诱导对牛奶蛋白的全身体液和细胞反应。T细胞反应随后下降，支持口服耐受的概念。9个月龄后接触牛奶蛋白会导致对这些蛋白的细胞和体液反应性降低。
我们的结果支持这样一种观点，即人类口服耐受的诱导是一种年龄依赖性现象。
