Eigenmann P A, Belli D C, Lüdi F, Kahn J M, Polla B S
Pediatric Clinic, University Hospital of Geneva, Switzerland.
J Allergy Clin Immunol. 1995 Oct;96(4):549-57. doi: 10.1016/s0091-6749(95)70300-4.
Discordant results have been reported about the role of lymphocyte proliferation assays in patients with cow's milk allergy. We studied the peripheral blood mononuclear cell response of 10 children with cow's milk allergy by means of a lymphocyte proliferation test to determine the diagnostic value of this assay, the clinical tolerance of a new therapeutic hydrolyzed formula, and the evolution of lymphocyte proliferation after 3 months of a cow's milk-free diet with the hydrolyzed formula. The lymphocyte proliferation at the time of diagnosis in the patients with cow's milk allergy was not statistically different from the results in the control group. The proliferation test performed after 3 months of diet with the hydrolyzed formula and restriction of cow's milk protein showed that the cellular proliferation remained globally the same compared with the proliferation at the time of diagnosis. The hydrolyzed formula proteins induced a lower cellular proliferation than milk proteins in patients with cow's milk allergy. Our results suggest that the lymphocyte proliferation test cannot be recommended for diagnostic purposes. However, in patients with cow's milk allergy the proliferation test affirmed the absence of immunogenicity of the hydrolyzed formula because it induced no significant T-cell activation.
关于淋巴细胞增殖试验在牛奶过敏患者中的作用，已有不一致的结果报道。我们通过淋巴细胞增殖试验研究了10名牛奶过敏儿童的外周血单个核细胞反应，以确定该试验的诊断价值、一种新型治疗性水解配方奶粉的临床耐受性，以及采用水解配方奶粉进行3个月无牛奶饮食后淋巴细胞增殖的变化情况。牛奶过敏患者诊断时的淋巴细胞增殖与对照组结果无统计学差异。采用水解配方奶粉并限制牛奶蛋白饮食3个月后进行的增殖试验表明，与诊断时的增殖情况相比，细胞增殖总体上保持不变。在牛奶过敏患者中，水解配方奶粉蛋白诱导的细胞增殖低于牛奶蛋白。我们的结果表明，淋巴细胞增殖试验不推荐用于诊断目的。然而，在牛奶过敏患者中，增殖试验证实了水解配方奶粉无免疫原性，因为它未诱导明显的T细胞活化。
