Gillespie S H, Ainscough S, Dickens A, Lewin J
Department of Microbiology, Royal Free Hospital School of Medicine, London.
J Med Microbiol. 1996 Jan;44(1):35-40. doi: 10.1099/00222615-44-1-35.
Phosphorylcholine (PC)-containing antigens were sought in 269 bacterial isolates from the mouth and respiratory tract by an enzyme immunoassay method. Only 41 (15%) isolates were PC-positive and of these 29 (70%) were strains of Haemophilus influenzae. Other species that produced positive results included two of five isolates of Gemella haemolysans, two of five isolates of Micrococcus spp., and a single strain each of Bacillus sp., Corynebacterium jeikeium, Lactococcus sp. and H. parainfluenzae. The presence of PC-containing antigens in H. influenzae may be an important source of cross-reaction in antigen detection techniques that detect the C-polysaccharide antigen of Streptococcus pneumoniae in respiratory specimens and would result in false positive results.
采用酶免疫测定法在269株来自口腔和呼吸道的细菌分离株中寻找含磷酰胆碱（PC）的抗原。只有41株（15%）分离株PC呈阳性，其中29株（70%）为流感嗜血杆菌菌株。产生阳性结果的其他菌种包括5株溶血孪生球菌中的2株、5株微球菌属中的2株，以及芽孢杆菌属、杰氏棒状杆菌、乳酸球菌属和副流感嗜血杆菌各1株。流感嗜血杆菌中含PC抗原的存在可能是抗原检测技术中交叉反应的一个重要来源，这些技术在呼吸道标本中检测肺炎链球菌的C多糖抗原时会导致假阳性结果。
