Hyttinen J M, Jansson K
Division of Environmental Health, National Public Health Institute, Kuopio, Finland.
Mutat Res. 1995 Dec;348(4):183-6. doi: 10.1016/0165-7992(95)90007-1.
3-Chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) is a potent direct-acting mutagen found in chlorinated drinking water. In the present study, the induction of DNA strand breaks and apurinic/apyrimidinic (AP) sites by MX in supercoiled PM2 DNA was examined using exonuclease III, which specifically cleaves the DNA at AP sites. The results showed that MX induced AP sites in great excess of direct strand breaks. In view of the known mutagenicity of AP sites, these results provide insight into the mechanism of MX-induced mutagenesis.
3-氯-4-（二氯甲基）-5-羟基-2（5H）-呋喃酮（MX）是一种在氯化饮用水中发现的强效直接作用诱变剂。在本研究中，使用核酸外切酶III检测了MX在超螺旋PM2 DNA中诱导的DNA链断裂和脱嘌呤/脱嘧啶（AP）位点，核酸外切酶III可特异性地在AP位点切割DNA。结果表明，MX诱导的AP位点大大超过直接链断裂。鉴于AP位点已知的致突变性，这些结果为MX诱导诱变的机制提供了见解。
