Kinbara H, Cunha G R
Department of Urology, Mie University School of Medicine, Japan.
Prostate. 1996 Jan;28(1):58-64. doi: 10.1002/(SICI)1097-0045(199601)28:1<58::AID-PROS8>3.0.CO;2-K.
Given the lobar complexity of the rat prostate at the morphological level, differences in secretory protein expression were investigated in individual prostatic ducts that constitute the subdivisions of the dorsal-lateral prostate, ie., the dorsal prostate, lateral prostate type 1 and lateral prostate type 2. For this purpose, individual prostatic ducts were microdissected from these prostatic lobes, photographed, and secretions subsequently collected from individual prostatic ducts and analyzed by Western blot for the expression of DP-1 and probasin, two major proteins expressed in rat the dorsal-lateral prostate. Many individual glands constituting the dorsal prostate, lateral prostate type 1 and lateral prostate type 2 co-express DP-1 and probasin, but at vastly different levels. DP-1 is a major secretory protein of the dorsal prostate and lateral prostate type 1, while probasin is the major secretory protein of the lateral prostate type 2. A small percentage of individual ducts of the dorsal prostate, lateral prostate type 1 and lateral prostate type 2 express either DP-1 or probasin. However, most of the individual prostatic ducts constituting the dorsal prostate and lateral prostate type 1 express DP-1 at high levels and probasin at low levels. Conversely, most of the individual prostatic glands that constitute the lateral prostate type 2 express probasin at high levels and DP-1 at low levels. This study emphasizes the morphological and functional heterogeneity within the prostate gland.
鉴于大鼠前列腺在形态学水平上的叶状复杂性，我们研究了构成背外侧前列腺各亚区（即背侧前列腺、1型外侧前列腺和2型外侧前列腺）的单个前列腺导管中分泌蛋白表达的差异。为此，从这些前列腺叶中显微切割出单个前列腺导管，拍照，随后从单个前列腺导管中收集分泌物，并通过蛋白质免疫印迹法分析大鼠背外侧前列腺中表达的两种主要蛋白质DP-1和前列腺素的表达情况。构成背侧前列腺、1型外侧前列腺和2型外侧前列腺的许多单个腺体共表达DP-1和前列腺素，但表达水平差异很大。DP-1是背侧前列腺和1型外侧前列腺的主要分泌蛋白，而前列腺素是2型外侧前列腺的主要分泌蛋白。背侧前列腺、1型外侧前列腺和2型外侧前列腺中有一小部分单个导管只表达DP-1或前列腺素。然而，构成背侧前列腺和1型外侧前列腺的大多数单个前列腺导管高水平表达DP-1，低水平表达前列腺素。相反，构成2型外侧前列腺的大多数单个前列腺腺体高水平表达前列腺素，低水平表达DP-1。这项研究强调了前列腺腺体内的形态学和功能异质性。
