Hayashi N, Sugimura Y, Kawamura J, Donjacour A A, Cunha G R
Department of Urology, Mie University, School of Medicine, Japan.
Biol Reprod. 1991 Aug;45(2):308-21. doi: 10.1095/biolreprod45.2.308.
Ductal morphogenesis and adult ductal branching patterns were examined in the rat prostate by a microdissection method. The rat prostate consists of paired (right and left) subdivisions which correspond in large part to the classically defined lobes: ventral prostate, lateral prostate, dorsal prostate, and coagulating gland. Of particular interest was the finding that the lateral prostate consists of two different ductal zones: (1) lateral type 1 prostate with 5-7 long main ducts (resembling miniature palm trees) that extend cranially towards both the seminal vesicle and dorsal prostate to arborize near the bladder neck, and (2) lateral type 2 prostate with 5-6 short main ducts that arborize caudal to the bladder neck and give rise to compact bushy glands. Both lateral prostatic groups had a ductal-acinar organization. The adult structure of the other rat prostatic lobes was also examined, and closely resembled their mouse counterparts. The ventral prostate, which had 2-3 pairs of slender main ducts per side, and the coagulating gland, which had 1 main duct per side, was completely ductal in structure. In contrast, the dorsal prostate, which had 5-6 pairs of main ducts per side, had a ductal-acinar structure. Ductal branching morphogenesis occurred at different rates in different lobes and was essentially complete in the prostate at the 30 days. Immunocytochemical studies with an antibody to DP-1, a major secretory protein of the rat dorsal prostate, revealed that secretory function was initiated at approximately 30 days after birth in the coagulating gland, the dorsal prostate, and lateral type 1 prostate. A consistent feature of the lateral type 2 prostate was the absence of DP-1. On Western blots, DP-1 was detected in the secretion of the coagulating gland, lateral type 1 and dorsal prostate, but not in the ventral and lateral type 2 prostate. Polyacrylamide gel electrophoresis confirmed this result and demonstrated that the lateral type 2 prostate expressed several low-molecular weight secretory proteins not found in the other lobes of the prostate. On the whole, the rat prostate exhibited considerable heterogeneity both between and within lobes in developmental processes, ductal patterning, histology, and functional expression.
采用显微解剖方法研究了大鼠前列腺的导管形态发生及成年导管分支模式。大鼠前列腺由成对的（右侧和左侧）分区组成，这些分区在很大程度上对应于经典定义的叶：腹侧前列腺、外侧前列腺、背侧前列腺和凝固腺。特别有趣的是发现外侧前列腺由两个不同的导管区组成：（1）外侧1型前列腺，有5 - 7条长的主导管（类似于微型棕榈树），向头侧延伸至精囊和背侧前列腺，在膀胱颈附近分支；（2）外侧2型前列腺，有5 - 6条短的主导管，在膀胱颈尾侧分支，形成紧密的浓密腺体。两个外侧前列腺组均具有导管 - 腺泡组织结构。还研究了其他大鼠前列腺叶的成年结构，其与小鼠的对应结构非常相似。腹侧前列腺每侧有2 - 3对细长的主导管，凝固腺每侧有1条主导管，结构完全为导管型。相比之下，背侧前列腺每侧有5 - 6对主导管，具有导管 - 腺泡结构。导管分支形态发生在不同叶中以不同速率发生，在30天时前列腺中基本完成。用针对大鼠背侧前列腺主要分泌蛋白DP - 1的抗体进行免疫细胞化学研究表明，凝固腺、背侧前列腺和外侧1型前列腺在出生后约30天开始分泌功能。外侧2型前列腺的一个一致特征是缺乏DP - 1。在蛋白质免疫印迹法中，在凝固腺、外侧1型和背侧前列腺的分泌物中检测到DP - 1，但在腹侧和外侧2型前列腺中未检测到。聚丙烯酰胺凝胶电泳证实了这一结果，并表明外侧2型前列腺表达了其他前列腺叶中未发现的几种低分子量分泌蛋白。总体而言，大鼠前列腺在发育过程、导管模式、组织学和功能表达方面，叶间和叶内均表现出相当大的异质性。
