Basset L, Ennamany R, Portail J P, Kretz O, Deffieux G, Badoc A, Guillemain B, Creppy E E
University of Bordeaux 2, Faculty of Pharmaceutical Sciences, Laboratory of Toxicology and Applied Hygiene, France.
Toxicology. 1995 Nov 30;103(2):121-5. doi: 10.1016/0300-483x(95)03113-t.
Bolesatine, a toxic protein isolated from Boletus satanas Lenz inhibits in vitro protein synthesis in a concentration-dependent manner in a cell line from a radiation-induced thymic lymphosarcoma (SP2/O) with a 50% inhibitory concentration (IC50) of 9.5 nM (0.6 microgram/ml). In vivo, an i.p. single injection of bolesatine, corresponding to 1/6 and 1/10 of 24-h 50% lethal dose, in Balb/c mice having ascitic tumour induced by the i.p. preinjection of SP2/O cells allows a remission of 50% and 30%, respectively. Treated mice survived 120 days after the treatment, i.e. 90 days after the death of control animals.
从撒旦牛肝菌中分离出的有毒蛋白博来毒素，能以浓度依赖的方式抑制辐射诱导的胸腺淋巴肉瘤细胞系（SP2/O）的体外蛋白质合成，其50%抑制浓度（IC50）为9.5 nM（0.6微克/毫升）。在体内实验中，给预先经腹腔注射SP2/O细胞诱导产生腹水瘤的Balb/c小鼠腹腔单次注射相当于24小时50%致死剂量的1/6和1/10的博来毒素，分别可使50%和30%的小鼠病情缓解。经治疗的小鼠在治疗后存活了120天，即比对照动物死亡时间晚90天。
