Ambrus G, Ilköy E, Jekkel A, Horváth G, Böcskei Z
Institute for Drug Research, Budapest, Hungary.
Steroids. 1995 Sep;60(9):621-5. doi: 10.1016/0039-128x(95)00077-4.
The genetically modified Mycobacterium sp. BCS 396 strain has been used to transform sterols with stigmastane side chain in order to obtain 26-oxidized metabolites. beta-Sitosterol (I) was transformed to 4-stigmasten-3-one (II), 26-hydroxy-4-stigmasten-3-one (III), and 3-oxo-4-stigmasten-26-oic acid (IV), while stigmasterol (V) was converted to 4,22-stigmastadien-3-one (VI), 6 beta-hydroxy-4,22-stigmastadien-3-one (VII), 26-hydroxy-4,22-stigmastadien-3-one (VIII), 3-oxo-4,22-stigmastadien-26-oic acid methyl ester (IX), and 3-oxo-1,4,22-stigmastatrien-26-oic acid methyl ester (X) with that strain. In both beta-sitosterol and stigmasterol, 26-oxidation generates the R-configuration on C-25.
转基因分枝杆菌属BCS 396菌株已被用于转化具有豆甾烷侧链的甾醇，以获得26-氧化代谢产物。β-谷甾醇（I）被转化为4-豆甾烯-3-酮（II）、26-羟基-4-豆甾烯-3-酮（III）和3-氧代-4-豆甾烯-26-酸（IV），而豆甾醇（V）则被该菌株转化为4,22-豆甾二烯-3-酮（VI）、6β-羟基-4,22-豆甾二烯-3-酮（VII）、26-羟基-4,22-豆甾二烯-3-酮（VIII）、3-氧代-4,22-豆甾二烯-26-酸甲酯（IX）和3-氧代-1,4,22-豆甾三烯-26-酸甲酯（X）。在β-谷甾醇和豆甾醇中，26-氧化在C-25上产生R-构型。
