Kukreja A, Walker C, Fitzmaurice T, Awan A, Love D N, Whalley J M, Field H J
Centre for Veterinary Science, Cambridge University Veterinary School, UK.
Vet Microbiol. 1998 Aug 15;62(4):303-11. doi: 10.1016/s0378-1135(98)00211-9.
In an attempt to determine if pregnant mice could be protected from abortion subsequent to challenge with equine herpesvirus-1 (EHV-1) in the mouse model of EHV-1 disease, female BALB/c mice were inoculated with baculovirus-expressed EHV-1 glycoprotein B (bac-gB), wild-type baculovirus (bac-wt), rabbit kidney (RK-13) or baby hamster kidney (BHK-21) cells. Using an ELISA, antibodies against EHV-1 were detected in the serum of mice following two injections of bac-gB and were enhanced by a third injection, after which low levels of neutralising antibody were also detected. After mating, mice in the bac-gB, bac-wt and RK-immunised groups were infected intranasally with 10(7) pfu of EHV-1 on day 16 of pregnancy. All challenged mice experienced body weight loss post-infection (pi). However, postnatally, the gB-immunised group demonstrated body weight gain which was not seen in the other groups. There were no maternal deaths in the gB-immunised group but 1/6 bac-wt-immunised and 3/6 RK-immunised mice died post-challenge. Litter survival rate was significantly higher (p < 0.001) for the gB-immunised dams (54%) than that of either the bac-wt-(9%) or RK-immunised (0%) dams and the mean body weight of young from the surviving bac-wt-immunised litter was significantly (p = 0.021) lower than either the gB-immunised group or the BHK-immunised unchallenged group at 10 days of age. The virus was not isolated from any foetus from a gB-immunised dam. However, the virus was detected in 9% of foetuses from bac-wt-immunised and 21% of foetuses from RK-immunised dams.
为了确定在马疱疹病毒1型（EHV-1）疾病的小鼠模型中，怀孕小鼠在受到EHV-1攻击后是否能免受流产影响，将雌性BALB/c小鼠接种杆状病毒表达的EHV-1糖蛋白B（bac-gB）、野生型杆状病毒（bac-wt）、兔肾（RK-13）或幼仓鼠肾（BHK-21）细胞。使用酶联免疫吸附测定法（ELISA），在两次注射bac-gB后，在小鼠血清中检测到针对EHV-1的抗体，第三次注射后抗体增强，之后还检测到低水平的中和抗体。交配后，在怀孕第16天，将bac-gB、bac-wt和RK免疫组的小鼠经鼻内接种10(7) 个噬斑形成单位（pfu）的EHV-1。所有受攻击的小鼠在感染后（pi）体重均下降。然而，产后，gB免疫组的小鼠体重增加，而其他组未见此现象。gB免疫组没有母鼠死亡，但1/6的bac-wt免疫和3/6的RK免疫小鼠在攻击后死亡。gB免疫母鼠的产仔存活率（54%）显著高于（p < 0.001）bac-wt免疫母鼠（9%）或RK免疫母鼠（0%），并且在10日龄时，存活的bac-wt免疫产仔的幼崽平均体重显著低于（p = 0.021）gB免疫组或BHK免疫未受攻击组。未从gB免疫母鼠的任何胎儿中分离到病毒。然而，在bac-wt免疫母鼠的9%胎儿和RK免疫母鼠的21%胎儿中检测到病毒。
