Lilja G, Magnusson C G, Kusoffsky E, Johansson S G, Oman H
Sachs' Children's Hospital, Stockholm, Sweden.
Allergy. 1995 Sep;50(9):723-8. doi: 10.1111/j.1398-9995.1995.tb01213.x.
Serum IgA and IgE levels were studied in the postnatal period in 21 infants having a paternal heredity of atopic disease. Three different sampling techniques were used, aspirated cord blood (CB), gravity-collected cord blood, and capillary collected blood at 4-5 days of age. Significant differences among the three sampling techniques were recorded for IgA (P < 0.01), but not for IgE. The IgA levels decreased from birth to 4-5 days of age in 90% (19/21 of infants (P < 0.01). The corresponding decrease in IgE levels was 20%. This postnatal difference in the frequency of decreasing/increasing IgA and IgE levels was significant (P < 0.05). An analysis of CB IgA to detect maternal contamination of CB was found to be of questionable value, since only 50% (2/4) of the cases with an elevated CB-IgA level could be considered contaminated. The results of this study further emphasize that aspiration of CB and capillary collection of blood at 4-5 days of age are the best sampling techniques to avoid contamination. The general finding that paternal heredity had no significant influence on infant IgE contrasts with the strong influence of maternal heredity. Further studies will show whether the explanation lies in genetic or transplacental factors, or in both kinds of factors.
对21名有特应性疾病父系遗传的婴儿在出生后进行血清IgA和IgE水平研究。采用了三种不同的采样技术，即抽取的脐带血（CB）、重力采集的脐带血以及出生4 - 5天时采集的毛细血管血。三种采样技术在IgA水平上有显著差异（P < 0.01），但IgE水平无显著差异。90%（21名婴儿中的19名）的婴儿IgA水平从出生到出生4 - 5天时下降（P < 0.01）。IgE水平相应下降20%。IgA和IgE水平下降/上升频率的这种出生后差异具有统计学意义（P < 0.05）。对CB IgA进行分析以检测CB是否受母体污染，其价值存疑，因为CB - IgA水平升高的病例中只有50%（4例中的2例）可被认为受污染。本研究结果进一步强调，抽取脐带血和在出生4 - 5天时采集毛细血管血是避免污染的最佳采样技术。父系遗传对婴儿IgE无显著影响这一总体发现与母系遗传的强烈影响形成对比。进一步的研究将表明解释是在于遗传因素还是经胎盘因素，抑或是两种因素都有。
