Garcia C, Arias M, Villar J M
Departmento de Biología Celular y Anatomía, facultad de Veterinaria, Universidad de León, España.
Anat Histol Embryol. 1995 Sep;24(3):145-7. doi: 10.1111/j.1439-0264.1995.tb00026.x.
A study is made of the morphological characteristics of the endodermic cells of the stage 5 chick embryo by means of in vitro cell culture techniques. The scanning electron microscope revealed that the endodermic cells in cultures were rounded, tended to be smooth and had few blebs and microvilli. Regarding cell projections typical of culture cells, such as filopodia, lamellipodia and pseudopodia, there was a noteworthy scarcity after 12 h growth, although greater cellular activity was observed at 24 h, characterized by the presence of filopodia and an ability of the cells to form clusters on the substratum. These facts show the morphological and adhesion movements of the endodermal cells studied to be related mainly with the presence of filopodia as the most abundant cell projections.
运用体外细胞培养技术，对5期鸡胚内胚层细胞的形态特征进行了研究。扫描电子显微镜显示，培养中的内胚层细胞呈圆形，表面趋于光滑，几乎没有泡状突起和微绒毛。关于培养细胞典型的细胞突起，如丝状伪足、片状伪足和伪足，在生长12小时后明显稀少，不过在24小时时观察到细胞活性增强，其特征是出现丝状伪足以及细胞能够在基质上形成细胞簇。这些事实表明，所研究的内胚层细胞的形态和黏附运动主要与丝状伪足这种最丰富的细胞突起的存在有关。
