Cecchi C, Pieri A, Nassi P, Fanelli A, Liguri G
Department of Biochemical Science, University of Florence, Italy.
Eur J Clin Chem Clin Biochem. 1995 Aug;33(8):519-24. doi: 10.1515/cclm.1995.33.8.519.
A new method for the determination of haemoglobin concentration in human whole blood has been devised. The assay takes advantage of the lysis of erythrocytes that occurs when blood penetrates the pores of a microporous membrane by a strong capillary effect. The released haemoglobin reacts with the reagents previously dried on the membrane, diffuses through the pores of the membrane and produces a uniform coloured spot whose reflectance at 556 nm can be evaluated at the side to that of application of the sample. In the experimental conditions used by authors, a linear relationship was obtained when the reciprocal reflectance was plotted against the haemoglobin concentration. Data obtained by the reflectometric method were compared with reference values on the same samples from centralized laboratories. Statistical analysis gave a correlation coefficient of 0.985 and only 4% of all haemoglobin determinations were outside the 95% confidence interval. No interference was observed by haematocrit, erythrocyte count, mean corpuscular volume or mean corpuscular haemoglobin concentration and leukocyte count. This method proved to be useful for rapid, accurate and precise assay of total haemoglobin on both capillary and venous blood.
已设计出一种测定人全血中血红蛋白浓度的新方法。该测定利用了血液通过强毛细作用穿透微孔膜孔时发生的红细胞裂解。释放出的血红蛋白与先前干燥在膜上的试剂发生反应，通过膜孔扩散并产生一个均匀的有色斑点，其在556nm处的反射率可在样品施加一侧进行评估。在作者使用的实验条件下，将倒数反射率与血红蛋白浓度作图时得到了线性关系。将反射法获得的数据与来自集中实验室的相同样品的参考值进行了比较。统计分析得出相关系数为0.985，所有血红蛋白测定中只有4%超出95%置信区间。未观察到血细胞比容、红细胞计数、平均红细胞体积或平均红细胞血红蛋白浓度以及白细胞计数的干扰。该方法被证明可用于快速、准确和精确地测定毛细血管血和静脉血中的总血红蛋白。
