评估HemoCue血浆血红蛋白分析仪在储存期间对红细胞浓缩物溶血情况的评估能力。

Evaluation of the HemoCue plasma haemoglobin analyser for assessing haemolysis in red cell concentrates during storage.

作者信息

Cardigan R, Smith K

机构信息

National Blood Service, Brentwood, UK.

出版信息

Vox Sang. 2002 Feb;82(2):76-9. doi: 10.1046/j.0042-9007.2001.00149.x.

DOI:10.1046/j.0042-9007.2001.00149.x

PMID:11906671

Abstract

BACKGROUND AND OBJECTIVES

To evaluate the HemoCue plasma haemoglobin (Hb) analyser for determining supernatant Hb in red cell concentrates (RCC) during storage and compare this to a spectrophotometric method.

MATERIALS AND METHODS

Samples from RCC (n = 20) at days 0, 7, 21, 35 and 42 of storage were tested by both methods for supernatant Hb.

RESULTS

Results are given as median with range. There was a significant correlation (r = 0.97, P < 0.0001), but statistically significant difference between the two methods (0.72 (0.36-2.77) spectrophotometric vs. 0.70 (0.3-2.6) g/l HemoCue). However, the mean bias was negligible at 0.06 g/l. The HemoCue method gave a interassay coefficient of variation of 5.6% at 1.2 g/l and was linear to at least 20 g/l.

CONCLUSION

The HemoCue plasma haemoglobin analyser is a simple, rapid, reliable and accurate method for the determination of supernatant Hb in RCC at the end of storage and compares well to established methods for supernatant Hb measurement.

摘要

背景与目的

评估HemoCue血浆血红蛋白（Hb）分析仪在储存期间测定红细胞浓缩液（RCC）中上清液Hb的性能，并与分光光度法进行比较。

材料与方法

采用两种方法对储存第0、7、21、35和42天的RCC样本（n = 20）进行上清液Hb检测。

结果

结果以中位数及范围表示。两种方法之间存在显著相关性（r = 0.97，P < 0.0001），但存在统计学显著差异（分光光度法为0.72（0.36 - 2.77）g/l，HemoCue法为0.70（0.3 - 2.6）g/l）。然而，平均偏差可忽略不计，为0.06 g/l。HemoCue法在1.2 g/l时批间变异系数为5.6%，线性范围至少达20 g/l。