Canalias F, Saco Y, Gella F J
Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Spain.
Eur J Clin Chem Clin Biochem. 1995 Aug;33(8):535-9.
Thirteen kits from different suppliers for measurement of creatine kinase activity in human serum according to the IFCC recommendations were analyzed and compared. Concentrations of AMP, ADP, creatine phosphate, glucose, magnesium ion, NADP+, glucose-6-phosphate dehydrogenase, hexokinase and pH were measured in the reagents by various analytical techniques and compared with those recommended b the IFCC. We also compared by regression analysis the results of creatine kinase catalytic concentration obtained in human sera using commercial kits and in-house prepared reagents according tot he IFCC recommendation. Creatine kinase was also measured in a reference material using the different reagents. The overall results of the activity measurements and the composition of the majority of the kits agree well with one another and with the IFCC recommendation. Minor deviations were found in the evaluation of a few kits. One kit yielded creatine kinase activity values that were 17% lower. Results obtained in the reference material measurements showed differences with some kits which were not found using human sera.
对13种来自不同供应商、按照国际临床化学和检验医学联合会（IFCC）推荐方法用于测定人血清中肌酸激酶活性的试剂盒进行了分析和比较。采用各种分析技术测定了试剂中AMP、ADP、磷酸肌酸、葡萄糖、镁离子、NADP⁺、葡萄糖-6-磷酸脱氢酶、己糖激酶的浓度以及pH，并与IFCC推荐的数值进行比较。我们还通过回归分析比较了使用商用试剂盒和按照IFCC推荐方法自行配制的试剂在人血清中获得的肌酸激酶催化浓度结果。使用不同试剂对一种参考物质中的肌酸激酶也进行了测定。活性测量的总体结果以及大多数试剂盒的组成相互之间以及与IFCC的推荐都非常吻合。在对少数试剂盒的评估中发现了微小偏差。有一种试剂盒得出的肌酸激酶活性值低了17%。在参考物质测量中获得的结果与某些试剂盒存在差异，而用人血清测量时未发现这些差异。
