Bonassi S, Bolognesi C, Abbondandolo A, Barale R, Bigatti P, Camurri L, Dalpra L, De Ferrari M, Forni A, Lando C
Servizio di Epidemiologia Ambientale, Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.
Cancer Epidemiol Biomarkers Prev. 1995 Sep;4(6):671-9.
The planning and evaluation of human cytogenetic studies should contemplate various confounders and effect modifiers, among these, sex and sex-related factors. The association between this variable and cytogenetic damage has been extensively studied, but conclusive evidence has thus far not been reached, especially for the most recent assays, such as the micronucleus test (MN). In the attempt to quantitatively estimate the sex effect on sister chromatid exchange (SCE), chromosomal aberration (CA), and MN in peripheral blood lymphocytes, we reanalyzed the original data sets of several biomonitoring studies performed over the last decades in 10 Italian laboratories. This approach yielded a very large database, namely 2140, 2495, and 2131 subjects screened for SCE, CA, and MN, respectively. Differences between sexes were expressed in terms of relative risk (RR) of females versus males, after adjustment for age, smoking habits, occupation exposure and inter- and intralaboratory variation. No difference between sexes was found for the frequency of SCE [RR = 1.01; 95% confidence interval (CI) = 0.99-1.03] and CA (RR = 1.00; 95% CI = 0.92-1.08) even if the CI of the RR for SCE includes the 3% excess in females frequently reported by the literature. Conversely, a 29% overall increase of the MN rate in females was observed in the whole data set (RR = 1.29; 95% CI = 1.20-1.38). Different trends by age of the MN rate are described in the two sexes, focusing on the peak observed in females in the menopausal period and on the subsequent decrease.
人类细胞遗传学研究的规划与评估应考虑各种混杂因素和效应修饰因素，其中包括性别及与性别相关的因素。该变量与细胞遗传学损伤之间的关联已得到广泛研究，但迄今尚未得出确凿证据，尤其是对于最新的检测方法，如微核试验（MN）。为了定量评估性别对外周血淋巴细胞中姐妹染色单体交换（SCE）、染色体畸变（CA）和MN的影响，我们重新分析了过去几十年在意大利10个实验室进行的几项生物监测研究的原始数据集。这种方法产生了一个非常大的数据库，即分别对2140、2495和2131名受试者进行了SCE、CA和MN检测。在对年龄、吸烟习惯、职业暴露以及实验室间和实验室内差异进行调整后，用女性相对于男性的相对风险（RR）来表示性别差异。SCE频率[RR = 1.01；95%置信区间（CI）= 0.99 - 1.03]和CA频率（RR = 1.00；95% CI = 0.92 - 1.08）在性别之间未发现差异，即使SCE的RR的CI包括文献中经常报道的女性3%的过量情况。相反，在整个数据集中观察到女性的MN率总体增加了29%（RR = 1.29；95% CI = 1.20 - 1.38）。描述了两性中MN率随年龄的不同趋势，重点关注女性在绝经期间观察到的峰值以及随后的下降情况。
