Ivanov V N, Svechnikova T A, Stabnikova E V, Gregirchak N N
Institute of Microbiological and Virology, Ukrainian Academy of Food Technologies, Kiev, Ukraine.
Mikrobiol Z. 1995 Jul-Aug;57(4):3-11.
Study of synchronous and asynchronous cultures of Bacillus megaterium, Bacillus thuringiensis and Bacillus licheniformis has shown that the duration of chromosomal DNA replication (period C) is proportional to the generation time, and time between two cycles of the DNA replication (known as period I). The duration of period C is nearly constant and makes up from 0.5 to 1.0 hour at the variations of the generation time from 1.5 to 2.75 hours. The duration of period B (the time between the termination of the cell division and initiation of DNA replication), and period D (the time between the termination of DNA replication and initiation of cell division) were experimentally revealed as stochastic parameters. The theoretical model of the bacterial cell cycle and the age structure of bacterial population was suggested. The main points of this theory are that periods C and I may be stochastically disposed in the division cycle of individual cells and a sum of duration of C- and I-periods is equal to generation time. The data calculated from the theoretical model were confirmed by the experimental data of flow cytofluorometric analysis of the age structure of synchronous and asynchronous cultures of the bacilli.
对巨大芽孢杆菌、苏云金芽孢杆菌和地衣芽孢杆菌同步培养物与异步培养物的研究表明，染色体DNA复制的持续时间（C期）与世代时间成正比，也与两个DNA复制周期之间的时间（称为I期）成正比。C期的持续时间几乎恒定，在世代时间从1.5小时变化到2.75小时时，C期持续时间为0.5至1.0小时。实验表明，B期（细胞分裂终止与DNA复制起始之间的时间）和D期（DNA复制终止与细胞分裂起始之间的时间）是随机参数。提出了细菌细胞周期和细菌群体年龄结构的理论模型。该理论的要点是，C期和I期可能随机分布在单个细胞的分裂周期中，且C期和I期的持续时间总和等于世代时间。通过对芽孢杆菌同步培养物和异步培养物年龄结构的流式细胞荧光分析实验数据，证实了从理论模型计算得出的数据。
