Nagy E, Szóke I, Gacs M, Csiszár K
Institute of Clinical Microbiology, Albert Szent-Györgyi University Medical School, Szeged, Hungary.
Acta Microbiol Immunol Hung. 1995;42(3):287-99.
Resistance to beta-lactam antibiotics of 183 clinical isolates belonging to Bacteroides, Porphyromonas and Prevotella was tested by a micro-broth dilution MIC method. Beta-lactamase production was screened by using nitrocefin sticks. Prevalence of resistance to different beta-lactam antibiotics was higher among the Bacteroides strains other than B. fragilis parallel with a beta-lactamase production (88% and 96%, respectively). Resistance was observed less frequently among Porphyromonas and Prevotella strains. Cefoxitin resistance was 11.5% and amoxicillin/clavulanic acid 3.5% among Bacteroides isolates, whereas no resistance was found to these antibiotics among the Porphyromonas and Prevotella strains. All strains tested were susceptible for imipenem. Beta-lactamase production of selected isolates was tested quantitatively. Beta-lactamase of B. fragilis 1 and B. levii differed in their isoelectric points, substrate profiles and inhibition by clavulanic acid, sulbactam and tazobactam.
采用微量肉汤稀释法对183株属于拟杆菌属、卟啉单胞菌属和普雷沃菌属的临床分离株进行了β-内酰胺类抗生素耐药性检测。使用硝基头孢菌素试纸条筛选β-内酰胺酶的产生情况。除脆弱拟杆菌外，拟杆菌属菌株对不同β-内酰胺类抗生素的耐药率较高，且与β-内酰胺酶的产生情况相关（分别为88%和96%）。在卟啉单胞菌属和普雷沃菌属菌株中观察到的耐药情况较少。在拟杆菌属分离株中，头孢西丁耐药率为11.5%，阿莫西林/克拉维酸耐药率为3.5%，而在卟啉单胞菌属和普雷沃菌属菌株中未发现对这些抗生素的耐药情况。所有检测菌株对亚胺培南均敏感。对选定分离株的β-内酰胺酶产生情况进行了定量检测。脆弱拟杆菌1型和二路拟杆菌的β-内酰胺酶在等电点、底物谱以及克拉维酸、舒巴坦和他唑巴坦对其的抑制作用方面存在差异。
