van den Berg E, Dijkhuizen T, Störkel S, Molenaar W M, de Jong B
Department of Medical Genetics, University of Groningen, The Netherlands.
Cancer Genet Cytogenet. 1995 Dec;85(2):152-4. doi: 10.1016/0165-4608(95)00035-6.
Chromosome aberrations were studied in short-term cultures of non-neoplastic renal tissue and tumor tissue in 60 patients, 41 male and 19 female, with renal cell cancer (RCC), and in normal renal parenchyma from two cases, one male and one female, at autopsy with non-kidney related disease. Cytogenetic analysis of the non-neoplastic renal tissue from the patents with RCC revealed clonal chromosome aberrations in 32 of 60 (53%) cases: -Y, +Y, +X, +5, +7, +10, +12, +13, +18, +21, and del(9)(q12). In all other cases, non-clonal numerical and structural chromosome aberrations were observed, except for three cases of culture failure. Forty-two cases of RCC yielded clonal chromosomal abnormalities. Trisomy 7 was present in 23 cases, loss of Y in 14, trisomy 5 and 12 in three, trisomy 18 in two cases, and trisomy 10 in one case. Clonal chromosomal anomalies found in two cases from kidneys without neoplasia were trisomy 7, trisomy 12, and trisomy 18 in one case, and monosomy of chromosome 15 in the other. The proportion of aberrant cells in both cases (with clonal and non-clonal aberrations) was, on average, 24%. Apparently, chromosomal abnormalities only play a role in the process of oncogenesis when they are in the appropriate stage and lineage of differentiation.
对60例肾细胞癌（RCC）患者（41例男性，19例女性）的非肿瘤性肾组织和肿瘤组织进行短期培养，研究染色体畸变情况，并对两例因非肾脏相关疾病尸检获得的正常肾实质（一例男性，一例女性）进行研究。对RCC患者的非肿瘤性肾组织进行细胞遗传学分析发现，60例中有32例（53%）存在克隆性染色体畸变：-Y、+Y、+X、+5、+7、+10、+12、+13、+18、+21和del(9)(q12)。在所有其他病例中，除三例培养失败外，均观察到非克隆性的数量和结构染色体畸变。42例RCC出现克隆性染色体异常。23例存在7号染色体三体，14例Y染色体缺失，3例存在5号和12号染色体三体，2例存在18号染色体三体，1例存在10号染色体三体。在两例无肿瘤的肾脏中发现的克隆性染色体异常，一例为7号、12号和18号染色体三体，另一例为15号染色体单体。在这两种情况（克隆性和非克隆性畸变）下，异常细胞的比例平均为24%。显然，染色体异常只有在处于适当的阶段和分化谱系时才会在肿瘤发生过程中发挥作用。
