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通过高分辨率核磁共振光谱法对新型VII型B族链球菌荚膜多糖进行结构解析。

Structural elucidation of the novel type VII group B Streptococcus capsular polysaccharide by high resolution NMR spectroscopy.

作者信息

Kogan G, Brisson J R, Kasper D L, von Hunolstein C, Orefici G, Jennings H J

机构信息

Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada.

出版信息

Carbohydr Res. 1995 Nov 7;277(1):1-9. doi: 10.1016/0008-6215(95)00195-y.

DOI:10.1016/0008-6215(95)00195-y
PMID:8548782
Abstract

The type VII capsular polysaccharide isolated from the newly discovered group B Streptococcus (GBS) strain contains D-glucose, D-galactose, N-acetyl-D-glucosamine and N-acetylneuraminic acid in the molar ratio 2:2:1:1. High-resolution one- and two-dimensional (1D and 2D) 1H and 13C NMR spectroscopy of the native and desialylated polysaccharides showed the type VII GBS capsular polysaccharide to contain the following branched hexasaccharide repeating unit: [formula: see text] Despite extensive structural similarity with the previously described GBS polysaccharides, the type VII polysaccharide showed no cross-reaction with the heterologous antisera.

摘要

从新发现的B族链球菌(GBS)菌株中分离出的VII型荚膜多糖含有摩尔比为2:2:1:1的D-葡萄糖、D-半乳糖、N-乙酰-D-葡萄糖胺和N-乙酰神经氨酸。天然和去唾液酸化多糖的高分辨率一维和二维(1D和2D)1H和13C NMR光谱表明,VII型GBS荚膜多糖含有以下分支六糖重复单元:[化学式:见正文]尽管与先前描述的GBS多糖在结构上有广泛的相似性,但VII型多糖与异源抗血清没有交叉反应。

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