Ko F N, Liao C H, Wu C L
Pharmacological Institute, College of Medicine, National Taiwan University, Taipei, Taiwan.
Chem Biol Interact. 1995 Nov 17;98(2):131-43. doi: 10.1016/0009-2797(95)03641-5.
The antioxidant effect of marchantinquinone was studied using various models. Marchantinquinone inhibited Fe(2+)-induced lipid peroxidation in rat brain homogenate in a concentration-dependent manner with an IC50 15.3 +/- 2.9 microM. Marchantinquinone was less effective than alpha-tocopherol and BHT in reducing the stable free radical diphenyl-2-picrylhydrazyl (DPPH). The IC0.200 were 37.5 +/- 2.5, 9.2 +/- 1.6 and 14.5 +/- 2.5 microM, respectively. Marchantinquinone also scavenged peroxyl radical derived from 2,2'-azobis(2-amidinopropane)dihydrochloride (AAPH) in aqueous system with a stoichiometric factor of 0.9 +/- 0.1. Furthermore, it prevented conjugated-diene formation and apolipoprotein B (apo B) oxidation of LDL. However, marchantinquinone did not chelate Fe2+ or possess superoxide anion and hydroxyl radical scavenging activity. It also did not scavenge 2,2'-azobis(2,4-dimethylvaleronitrile) (AMVN)-derived peroxyl radical in hexane. These results indicate that marchantinquinone may be an effective antioxidant and can protect rat brain homogenate and LDL against oxidation.
采用多种模型研究了 marchantinquinone 的抗氧化作用。Marchantinquinone 以浓度依赖方式抑制大鼠脑匀浆中 Fe(2+)诱导的脂质过氧化，IC50 为 15.3±2.9 μM。在还原稳定自由基二苯基 -2- 苦基肼 (DPPH) 方面，Marchantinquinone 的效果不如 α-生育酚和 BHT。其 IC0.200 分别为 37.5±2.5、9.2±1.6 和 14.5±2.5 μM。Marchantinquinone 还能清除水体系中由 2,2'-偶氮二异丁脒盐酸盐 (AAPH) 产生的过氧自由基，化学计量系数为 0.9±0.1。此外，它能防止 LDL 的共轭二烯形成和载脂蛋白 B (apo B) 氧化。然而，Marchantinquinone 不螯合 Fe2+，也不具有超氧阴离子和羟基自由基清除活性。它也不能清除己烷中由 2,2'-偶氮二(2,4-二甲基戊腈) (AMVN) 产生的过氧自由基。这些结果表明，Marchantinquinone 可能是一种有效的抗氧化剂，能够保护大鼠脑匀浆和 LDL 免受氧化。
