从半球叶苔中分离得到的苔色醌，作为脂质过氧化抑制剂和自由基清除剂。

Marchantinquinone, isolated from Reboulia hemisphaerica, as inhibitor of lipid peroxidation and as free radical scavenger.

作者信息

Ko F N, Liao C H, Wu C L

机构信息

Pharmacological Institute, College of Medicine, National Taiwan University, Taipei, Taiwan.

出版信息

Chem Biol Interact. 1995 Nov 17;98(2):131-43. doi: 10.1016/0009-2797(95)03641-5.

DOI:10.1016/0009-2797(95)03641-5

PMID:8548853

Abstract

The antioxidant effect of marchantinquinone was studied using various models. Marchantinquinone inhibited Fe(2+)-induced lipid peroxidation in rat brain homogenate in a concentration-dependent manner with an IC50 15.3 +/- 2.9 microM. Marchantinquinone was less effective than alpha-tocopherol and BHT in reducing the stable free radical diphenyl-2-picrylhydrazyl (DPPH). The IC0.200 were 37.5 +/- 2.5, 9.2 +/- 1.6 and 14.5 +/- 2.5 microM, respectively. Marchantinquinone also scavenged peroxyl radical derived from 2,2'-azobis(2-amidinopropane)dihydrochloride (AAPH) in aqueous system with a stoichiometric factor of 0.9 +/- 0.1. Furthermore, it prevented conjugated-diene formation and apolipoprotein B (apo B) oxidation of LDL. However, marchantinquinone did not chelate Fe2+ or possess superoxide anion and hydroxyl radical scavenging activity. It also did not scavenge 2,2'-azobis(2,4-dimethylvaleronitrile) (AMVN)-derived peroxyl radical in hexane. These results indicate that marchantinquinone may be an effective antioxidant and can protect rat brain homogenate and LDL against oxidation.

摘要

采用多种模型研究了 marchantinquinone 的抗氧化作用。Marchantinquinone 以浓度依赖方式抑制大鼠脑匀浆中 Fe(2+)诱导的脂质过氧化，IC50 为 15.3±2.9 μM。在还原稳定自由基二苯基 -2- 苦基肼 (DPPH) 方面，Marchantinquinone 的效果不如 α-生育酚和 BHT。其 IC0.200 分别为 37.5±2.5、9.2±1.6 和 14.5±2.5 μM。Marchantinquinone 还能清除水体系中由 2,2'-偶氮二异丁脒盐酸盐 (AAPH) 产生的过氧自由基，化学计量系数为 0.9±0.1。此外，它能防止 LDL 的共轭二烯形成和载脂蛋白 B (apo B) 氧化。然而，Marchantinquinone 不螯合 Fe2+，也不具有超氧阴离子和羟基自由基清除活性。它也不能清除己烷中由 2,2'-偶氮二(2,4-二甲基戊腈) (AMVN) 产生的过氧自由基。这些结果表明，Marchantinquinone 可能是一种有效的抗氧化剂，能够保护大鼠脑匀浆和 LDL 免受氧化。