Ko F N, Cheng Z J, Lin C N, Teng C M
Pharmacological Institute, College of Medicine, National Taiwan University, Taipei.
Free Radic Biol Med. 1998 Jul 15;25(2):160-8. doi: 10.1016/s0891-5849(98)00031-8.
The antioxidant properties of prenylflavones, isolated from Artocarpus heterophyllus Lam., was evaluated in this study. Among them, artocarpine, artocarpetin, artocarpetin A, and cycloheterophyllin diacetate and peracetate had no effect on iron-induced lipid peroxidation in rat brain homogenate. They also did not scavenge the stable free radical 1,1-diphenyl-2-picrylhydrazyl. In contrast, cycloheterophyllin and artonins A and B inhibited iron-induced lipid peroxidation in rat brain homogenate and scavenged 1,1-diphenyl-2-picrylhydrazyl. They also scavenged peroxyl radicals and hydroxyl radicals that were generated by 2,2'-azobis(2-amidinopropane) dihydrochloride and the Fe3+-ascorbate-EDTA-H2O2 system, respectively. However, they did not inhibit xanthine oxidase activity or scavenge superoxide anion, hydrogen peroxide, carbon radical, or peroxyl radicals derived from 2,2'-azobis(2,4-dimethylvaleronitrile) in hexane. Moreover, cycloheterophyllin and artonins A and B inhibited copper-catalyzed oxidation of human low-density lipoprotein, as measured by fluorescence intensity, thiobarbituric acid-reactive substance and conjugated-diene formations and electrophoretic mobility. It is concluded that cycloheterophyllin and artonins A and B serve as powerful antioxidants against lipid peroxidation when biomembranes are exposed to oxygen radicals.
本研究对从菠萝蜜（Artocarpus heterophyllus Lam.）中分离出的异戊烯基黄酮的抗氧化特性进行了评估。其中，菠萝蜜碱、菠萝蜜黄素、菠萝蜜黄素A以及环叶波罗蜜二乙酸酯和全乙酸酯对大鼠脑匀浆中铁诱导的脂质过氧化没有影响。它们也不能清除稳定自由基1,1-二苯基-2-苦基肼。相比之下，环叶波罗蜜素以及波罗蜜宁A和B可抑制大鼠脑匀浆中铁诱导的脂质过氧化，并能清除1,1-二苯基-2-苦基肼。它们还分别清除了由2,2'-偶氮二（2-脒基丙烷）二盐酸盐和Fe3+-抗坏血酸-EDTA-H2O2体系产生的过氧自由基和羟基自由基。然而，它们并不抑制黄嘌呤氧化酶的活性，也不能清除己烷中由2,2'-偶氮二（2,4-二甲基戊腈）产生的超氧阴离子、过氧化氢、碳自由基或过氧自由基。此外，通过荧光强度、硫代巴比妥酸反应性物质、共轭二烯形成以及电泳迁移率测定，环叶波罗蜜素以及波罗蜜宁A和B可抑制铜催化的人低密度脂蛋白氧化。结论是，当生物膜暴露于氧自由基时，环叶波罗蜜素以及波罗蜜宁A和B可作为强大的抗氧化剂抵抗脂质过氧化。
