Nelissen-Vrancken H J, Debets J J, Snoeckx L H, Daemen M J, Smits J F
Department of Pharmacology, University of Limburg, The Netherlands.
Circulation. 1996 Jan 15;93(2):349-55. doi: 10.1161/01.cir.93.2.349.
In the present study, we investigated the time dependency and regional differences of the vascular adaptation of the myocardium after myocardial infarction (MI) in rats.
MI was induced by total occlusion of the left anterior descending coronary artery. Time-dependent adaptation of the coronary vasculature was determined by histological staining of endothelial cells and measurement of basal and maximal coronary flow at days 0, 4, 7, 21, 35, and 90 after surgery in isolated retrogradely perfused hearts of sham-operated and infarcted rats. Cardiac function was determined during anterograde perfusion. In a separate group of experiments, regional myocardial flow was measured with radiolabeled microspheres in sham-operated and infarcted hearts to determine local differences in adaptation. Basal coronary flow was completely normalized within 7 days, whereas maximal coronary flow was not normalized until 35 days after MI. Normal growth, as observed in sham-operated hearts, resulted in a parallel increase in coronary flow and tissue mass from day 7 to 35 after surgery. In contrast, the increase in coronary flow was lower than the hypertrophic response in the right ventricles and septa of infarcted hearts, whereas a parallel increase in tissue mass and coronary flow was observed in the left ventricles of these hearts. These functional data were supported by structural data that showed the presence of numerous and dilated vessels, especially in the border zone of the infarcted and noninfarcted tissue.
These observations demonstrate that vessel growth, predominantly in the region adjacent to the infarcted zone, results in complete normalization of coronary vasodilatory capacity within 35 days after MI.
在本研究中，我们调查了大鼠心肌梗死后心肌血管适应的时间依赖性和区域差异。
通过完全闭塞左冠状动脉前降支诱导心肌梗死。在假手术和梗死大鼠的离体逆行灌注心脏中，于术后第0、4、7、21、35和90天，通过内皮细胞组织学染色以及基础和最大冠状动脉血流量的测量，确定冠状动脉血管系统的时间依赖性适应情况。在顺行灌注期间测定心功能。在另一组实验中，用放射性标记微球测量假手术和梗死心脏的区域心肌血流量，以确定适应的局部差异。基础冠状动脉血流量在7天内完全恢复正常，而最大冠状动脉血流量直到心肌梗死后35天才恢复正常。如在假手术心脏中观察到的正常生长，导致术后第7天至35天冠状动脉血流量和组织质量平行增加。相比之下，梗死心脏右心室和室间隔中冠状动脉血流量的增加低于肥厚反应，而在这些心脏的左心室中观察到组织质量和冠状动脉血流量平行增加。这些功能数据得到了结构数据的支持，结构数据显示存在大量扩张的血管，尤其是在梗死和未梗死组织的边界区域。
这些观察结果表明，血管生长主要发生在梗死区域附近，导致心肌梗死后35天内冠状动脉舒张能力完全恢复正常。
