肿瘤坏死因子-α诱导人气道高反应性

Induction of human airway hyperresponsiveness by tumour necrosis factor-alpha.

作者信息

Anticevich S Z, Hughes J M, Black J L, Armour C L

机构信息

Department of Pharmacy, University of Sydney, Australia.

出版信息

Eur J Pharmacol. 1995 Sep 15;284(1-2):221-5. doi: 10.1016/0014-2999(95)00463-u.

DOI:10.1016/0014-2999(95)00463-u

PMID:8549631

Abstract

Tumour necrosis factor-alpha (TNF alpha) is implicated in the pathogenesis of asthma; however, little is known of its direct effect on smooth muscle reactivity. We investigated the effect of TNF alpha on the responsiveness of human bronchial tissue to electrical field stimulation in vitro. Incubation of non-sensitized tissue with 1 nM, 3 nM and 10 nM TNF alpha significantly increased responsiveness to electrical field stimulation (113 +/- 8, 110 +/- 4 and 112 +/- 2% respectively) compared to control (99 +/- 2%) (P < 0.05, n = 6). Responses were not increased in sensitized tissue (101 +/- 3% versus 105 +/- 5%, n = 3, P > 0.05) nor were responses to exogenous acetylcholine (93 +/- 4% versus 73 +/- 7%, n = 3, P = 0.38). These results show that TNF alpha causes an increase in responsiveness of human bronchial tissue and that this occurs prejunctionally on the parasympathetic nerve pathway. This is the first report of a cytokine increasing human airway tissue responsiveness.

摘要

肿瘤坏死因子-α（TNFα）与哮喘的发病机制有关；然而，其对平滑肌反应性的直接影响却知之甚少。我们在体外研究了TNFα对人支气管组织电场刺激反应性的影响。与对照组（99±2%）相比，用1 nM、3 nM和10 nM TNFα孵育未致敏组织可显著增加对电场刺激的反应性（分别为113±8、110±4和112±2%）（P<0.05，n = 6）。致敏组织的反应性未增加（101±3%对105±5%，n = 3，P>0.05），对外源性乙酰胆碱的反应性也未增加（93±4%对73±7%，n = 3，P = 0.38）。这些结果表明，TNFα可导致人支气管组织反应性增加，且这种增加发生在副交感神经通路的节前。这是关于细胞因子增加人气道组织反应性的首次报道。