Styriak I, Conková E, Böhm J
Institute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovakia.
Folia Microbiol (Praha). 1994;39(6):579-81. doi: 10.1007/BF02814113.
Spores of Fusarium sacchari var. subglutinans isolated from broiler feed BR1 were obtained at an average concentration of 1.5/mg in 25% of tested samples. The spore concentration was increased from 1 to 100/mg of solid substrate (BR2; biscuit) or to 1/nL of Sabouraud broth after 3 weeks of cultivation. Mycotoxin analyses of these three substrates showed negative reactions for T-2 toxin and zearalenone but a positive reaction for deoxynivalenol (DON) which was found in concentrations of 5 ppm in Sabouraud broth, 50 ppm in BR2 and 220 ppm in biscuit. Therefore, our F. sacchari isolate appeared to be a DON producer.
从肉鸡饲料BR1中分离出的胶孢镰刀菌变种亚粘团孢的孢子，在25%的测试样本中平均浓度为1.5个/毫克。经过3周培养后，孢子浓度在固体基质（BR2；饼干）中从1个/毫克增加到100个/毫克，在沙氏肉汤中增加到1个/纳升。对这三种基质的霉菌毒素分析显示，T-2毒素和玉米赤霉烯酮呈阴性反应，但脱氧雪腐镰刀菌烯醇（DON）呈阳性反应；在沙氏肉汤中DON浓度为5 ppm，在BR2中为50 ppm，在饼干中为220 ppm。因此，我们分离出的胶孢镰刀菌似乎是DON的产生菌。
