Chung C W, Tigges M, Stone R A
Scheie Eye Institute, University of Pennsylvania School of Medicine, Philadelphia 19104-6075, USA.
Invest Ophthalmol Vis Sci. 1996 Jan;37(1):238-45.
To localize and characterize nerves in primate meibomian glands using immunohistochemical staining for neuropeptides and neuronal enzymes.
Upper eyelids were obtained from seven rhesus monkeys (Macaca mulatta) and one cynomolgous monkey (Macaca fascicularis). The tissues were fixed either by immersion in Zamboni's fixative or by transcardiac perfusion with paraformaldehyde and glutaraldehyde and were then postfixed. Cryostat tissue sections of the lids were stained by immunohistochemistry using rabbit antisera to neuron-specific enolase (NSE), tyrosine hydroxylase (TH), neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), calcitonin gene-related peptide (CGRP), and substance P (SP), followed by a fluorescence visualization system.
Used as a marker for the overall nerve distribution, NSE antibodies revealed abundant smooth and varicose nerve fibers closely apposed to the basement membranes of acini of the meibomian glands. Numerous nerve fibers near the meibomian gland acini were immunoreactive for NPY and VIP, but nerve fibers containing TH, CGRP, and SP were more sparse in the meibomian glands. Nerve fibers also were visualized in other eyelid structures, including conjunctiva, epidermis, hair follicles, and subconjunctival lymphoid follicles.
The meibomian glands of rhesus and cynomolgous monkeys are richly innervated by diverse nerve fiber types. The immunohistochemical staining suggests a largely parasympathetic origin for this innervation, with relatively smaller contributions from sympathetic and sensory sources. These findings also suggest that meibomian gland secretion is under the control of diverse neurotransmitter-neuromodulator mechanisms.
利用神经肽和神经元酶的免疫组织化学染色对灵长类睑板腺中的神经进行定位和特征描述。
从7只恒河猴（猕猴属）和1只食蟹猴（猕猴属）获取上眼睑。组织通过浸入赞博尼固定液或经心脏灌注多聚甲醛和戊二醛进行固定，然后进行后固定。眼睑的低温恒温器组织切片采用免疫组织化学方法，用兔抗神经元特异性烯醇化酶（NSE）、酪氨酸羟化酶（TH）、神经肽Y（NPY）、血管活性肠多肽（VIP）、降钙素基因相关肽（CGRP）和P物质（SP）的抗血清进行染色，随后使用荧光可视化系统。
作为整体神经分布的标志物，NSE抗体显示出丰富的平滑且有曲张的神经纤维，紧密贴附于睑板腺腺泡的基底膜。睑板腺腺泡附近的许多神经纤维对NPY和VIP呈免疫反应性，但含有TH、CGRP和SP的神经纤维在睑板腺中较为稀疏。在其他眼睑结构中也观察到了神经纤维，包括结膜、表皮、毛囊和结膜下淋巴滤泡。
恒河猴和食蟹猴的睑板腺由多种神经纤维类型丰富地支配。免疫组织化学染色表明这种支配在很大程度上源于副交感神经，交感神经和感觉神经的贡献相对较小。这些发现还表明睑板腺分泌受多种神经递质 - 神经调质机制的控制。
