菊欧文氏菌渗透保护剂转运蛋白基因ousA的特性分析
Characterization of the Erwinia chrysanthemi osmoprotectant transporter gene ousA.
作者信息
Gouesbet G, Trautwetter A, Bonnassie S, Wu L F, Blanco C
机构信息
Centre National de la Recherche Scientifique (CNRS) URA 256, Département Membranes et Osmorégulation, Université de Rennes I, France.
出版信息
J Bacteriol. 1996 Jan;178(2):447-55. doi: 10.1128/jb.178.2.447-455.1996.
Abstract
Growth of Erwinia chrysanthemi in media of elevated osmolarity can be achieved by the uptake and accumulation of various osmoprotectants. This study deals with the cloning and sequencing of the ousA gene-encoded osmoprotectant uptake system A from E. chrysanthemi 3937. OusA belongs to the superfamily of solute ion cotransporters. This osmotically inducible system allows the uptake of glycine betaine, proline, ectoine, and pipecolic acid and presents strong similarities in nucleotide sequence and protein function with the proline/betaine porter of Escherichia coli encoded by proP. The control of ousA expression is clearly different from that of proP. It is induced by osmotic strength and repressed by osmoprotectants. Its expression in E. coli is controlled by H-NS and is rpoS dependent in the exponential phase but unaffected by the stationary phase.
摘要
通过摄取和积累各种渗透保护剂，菊欧文氏菌可以在高渗透压培养基中生长。本研究涉及从菊欧文氏菌3937中克隆和测序由ousA基因编码的渗透保护剂摄取系统A。OusA属于溶质离子共转运蛋白超家族。这个渗透压诱导系统允许摄取甘氨酸甜菜碱、脯氨酸、四氢嘧啶和哌啶酸，并且在核苷酸序列和蛋白质功能上与大肠杆菌中由proP编码的脯氨酸/甜菜碱转运蛋白有很强的相似性。ousA表达的调控明显不同于proP。它由渗透压诱导，并被渗透保护剂抑制。它在大肠杆菌中的表达受H-NS控制，在指数生长期依赖rpoS，但在稳定期不受影响。
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