鼠伤寒沙门氏菌中鞭毛特异性抗σ因子FlgM输出的fliD、fliS和fliT负调控
Negative regulation by fliD, fliS, and fliT of the export of the flagellum-specific anti-sigma factor, FlgM, in Salmonella typhimurium.
作者信息
Yokoseki T, Iino T, Kutsukake K
机构信息
Faculty of Applied Biological Science, Hiroshima University, Japan.
出版信息
J Bacteriol. 1996 Feb;178(3):899-901. doi: 10.1128/jb.178.3.899-901.1996.
Abstract
The fliD operon of Salmonella typhimurium consists of three flagellar genes, fliD, fliS, and fliT, and is transcribed in this order. It has been shown that an fliD::Tn10 mutation causes an excess export of the flagellum-specific anti-sigma factor, FlgM, resulting in an overexpression of flagellar class 3 operons. In this study, using gene-disruption mutants in the individual genes in the fliD operon, we showed that mutations in any one of the genes in the operon enhanced both FlgM export and the expression of flagellar regulon. This indicates that all three genes in the operon are involved in the negative regulation of FlgM export.
摘要
鼠伤寒沙门氏菌的fliD操纵子由三个鞭毛基因fliD、fliS和fliT组成，并按此顺序转录。研究表明，fliD::Tn10突变会导致鞭毛特异性抗σ因子FlgM过度输出，从而导致鞭毛3类操纵子的过表达。在本研究中，我们利用fliD操纵子中各个基因的基因破坏突变体，证明操纵子中任何一个基因的突变都会增强FlgM的输出和鞭毛调节子的表达。这表明操纵子中的所有三个基因都参与了FlgM输出的负调控。
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