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来自两个启动子的转录及自身调控有助于控制鼠伤寒沙门氏菌鞭毛调控基因flgM的表达。

Transcription from two promoters and autoregulation contribute to the control of expression of the Salmonella typhimurium flagellar regulatory gene flgM.

作者信息

Gillen K L, Hughes K T

机构信息

Department of Microbiology, University of Washington, Seattle 98195.

出版信息

J Bacteriol. 1993 Nov;175(21):7006-15. doi: 10.1128/jb.175.21.7006-7015.1993.

DOI:10.1128/jb.175.21.7006-7015.1993
PMID:7693654
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC206828/
Abstract

The flgM gene product has been shown to be a negative regulator of flagellin transcription in Salmonella typhimurium (K. L. Gillen and K. T. Hughes, J. Bacteriol. 173:2301-2310, 6453-6459, 1991; K. Ohnishi, K. Kutsukake, H. Suzuki, and T. Iino, Mol. Microbiol. 6:3149-3157, 1992). Mud-lac fusions to the flgM gene were isolated and used to characterize the regulation of flgM gene expression. Transcription of the flgM gene was decreased more than 30-fold in strains with the flagellar master regulatory genes, flhC and flhD, deleted. A class 2 flagellar defect caused a slight increase of flgM gene transcription unless a wild-type copy of the flgM gene was present, in which case transcription was decreased threefold. A deletion in the gene for the alternative sigma factor sigma 28 (FliA) caused a fourfold decrease in flgM expression. Insertional inactivation of a gene upstream of the flgM gene (flgA) in a fliA mutant strain caused transcription of the flgM gene to be decreased to a basal level. Northern (RNA) blot analysis confirmed the presence of two transcripts through the flgM gene, one which initiates upstream of the flgM gene and a second which initiates upstream of the flgA gene.

摘要

flgM基因产物已被证明是鼠伤寒沙门氏菌中鞭毛蛋白转录的负调节因子（K. L. 吉伦和K. T. 休斯，《细菌学杂志》173:2301 - 2310, 6453 - 6459, 1991；大西健、久津岳、铃木浩和饭野哲，《分子微生物学》6:3149 - 3157, 1992）。分离出与flgM基因的Mud - lac融合体，并用于表征flgM基因表达的调控。在鞭毛主调控基因flhC和flhD缺失的菌株中，flgM基因的转录下降了30倍以上。2类鞭毛缺陷导致flgM基因转录略有增加，除非存在flgM基因的野生型拷贝，在这种情况下转录下降三倍。替代sigma因子sigma 28（FliA）基因的缺失导致flgM表达下降四倍。在fliA突变菌株中，flgM基因上游基因（flgA）的插入失活导致flgM基因的转录降至基础水平。Northern（RNA）印迹分析证实flgM基因存在两种转录本，一种在flgM基因上游起始，另一种在flgA基因上游起始。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2005/206828/e85d68b3d80c/jbacter00063-0290-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2005/206828/185a5c791ed3/jbacter00063-0285-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2005/206828/34b52b8f9616/jbacter00063-0288-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2005/206828/e85d68b3d80c/jbacter00063-0290-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2005/206828/185a5c791ed3/jbacter00063-0285-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2005/206828/34b52b8f9616/jbacter00063-0288-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2005/206828/e85d68b3d80c/jbacter00063-0290-a.jpg

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