古菌中的脱氧胸苷一磷酸生物合成
dTMP biosynthesis in Archaea.
作者信息
Nyce G W, White R H
机构信息
Department of Biochemistry and Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg 24061-0308, USA.
出版信息
J Bacteriol. 1996 Feb;178(3):914-6. doi: 10.1128/jb.178.3.914-916.1996.
Abstract
The biosynthesis of dTMP has been studied in cell extracts of two different members of the domain Archaea, Methanosarcina thermophila and Sulfolobus solfataricus. In M. thermophila, the dTMP was formed from dUMP and [methylene-2H2]-5,10-methylenetetrahydrosarcinapterin generated in situ from added [methylene-2H2] formaldehyde and the tetrahydrosarcinapterin present in the cell extract. In S. solfataricus, the 5,10-methyl-enetetrahydro derivative of a synthetic fragment of sulfopterin, the modified folate present in these cells, served as the C1 donor. These data indicate that the Archaea thymidylate synthases carry out the same basic reaction which occurs in other organisms but use the 5,10-methylenetetrahydro derivatives of modified folates as C1 donors.
摘要
已在古菌域的两个不同成员嗜热甲烷八叠球菌和嗜热栖热菌的细胞提取物中研究了dTMP的生物合成。在嗜热甲烷八叠球菌中，dTMP由dUMP和由添加的[亚甲基 - 2H2]甲醛与细胞提取物中存在的四氢萨金蝶呤原位生成的[亚甲基 - 2H2]-5,10 - 亚甲基四氢萨金蝶呤形成。在嗜热栖热菌中，这些细胞中存在的修饰叶酸——硫蝶呤的合成片段的5,10 - 亚甲基四氢衍生物用作C1供体。这些数据表明，古菌胸苷酸合酶进行的基本反应与其他生物体中发生的反应相同，但使用修饰叶酸的5,10 - 亚甲基四氢衍生物作为C1供体。
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