通过共表达植物酰基-酰基载体蛋白去饱和酶和铁氧化还原蛋白对大肠杆菌脂肪酸组成进行修饰。
Modification of the fatty acid composition of Escherichia coli by coexpression of a plant acyl-acyl carrier protein desaturase and ferredoxin.
作者信息
Cahoon E B, Mills L A, Shanklin J
机构信息
Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA.
出版信息
J Bacteriol. 1996 Feb;178(3):936-9. doi: 10.1128/jb.178.3.936-939.1996.
Abstract
Expression of a plant delta 6-palmitoyl (16:0)-acyl carrier protein desaturase in Escherichia coli resulted in the accumulation of the novel monounsaturated fatty acids delta 6-hexadecenoic acid (16:1 delta 6) and delta 8-octadecenoic acid. Amounts of 16:1 delta 6 accumulated by E. coli were increased more than twofold by the expression of a plant ferredoxin together with the delta 6-16:0-acyl carrier protein desaturase.
摘要
一种植物δ6-棕榈酰（16:0）-酰基载体蛋白去饱和酶在大肠杆菌中的表达导致了新型单不饱和脂肪酸δ6-十六碳烯酸（16:1δ6）和δ8-十八碳烯酸的积累。大肠杆菌积累的16:1δ6的量通过植物铁氧还蛋白与δ6-16:0-酰基载体蛋白去饱和酶一起表达而增加了两倍多。
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