Donaldson K, Golyasnya N
Department of Biological Sciences, Napier University, Edinburgh, U.K.
J Pathol. 1995 Nov;177(3):303-7. doi: 10.1002/path.1711770313.
This study utilized two samples of amosite asbestos which differ in their length, but not in their diameter and which have been shown previously to have very different abilities to cause pathology in rats exposed by instillation or inhalation. The activity of these amosite samples in causing chromosomal aberrations in Chinese hamster ovary cells in culture was examined, along with the effect of the glutathione (GSH) synthesis-inhibiting agent buthionine sulphoximine. The incidence of chromosomal aberrations in cells treated with the short fibre sample was similar to control levels; the long amosite sample caused significantly more chromosomal aberrations than the short fibre sample. When cells were treated with buthionine sulphoximine to decrease the levels of intracellular glutathione, the incidence of chromosomal aberrations was increased in the control cells, but also on treatment with both short and long amosite, the long sample again being considerably more active than the short. The pathogenicity of the long amosite may result from the ability of the fibres to cause chromosome damage, while the enhancement of this damage caused by decreasing intracellular glutathione suggests that the asbestos fibres may impose an oxidant stress on the cells which contributes to these aberrations.
本研究使用了两种铁石棉样本，它们的长度不同，但直径相同，并且先前已表明，通过滴注或吸入方式使大鼠接触这两种样本后，它们导致病变的能力差异很大。研究了这些铁石棉样本在体外培养的中国仓鼠卵巢细胞中引起染色体畸变的活性，以及谷胱甘肽（GSH）合成抑制剂丁硫氨酸亚砜胺的影响。用短纤维样本处理的细胞中染色体畸变的发生率与对照水平相似；长纤维铁石棉样本引起的染色体畸变明显多于短纤维样本。当用丁硫氨酸亚砜胺处理细胞以降低细胞内谷胱甘肽水平时，对照细胞中染色体畸变的发生率增加，而且在用长短两种铁石棉处理的细胞中，长纤维样本的活性再次明显高于短纤维样本。长纤维铁石棉的致病性可能源于纤维引起染色体损伤的能力，而细胞内谷胱甘肽水平降低导致这种损伤加剧，这表明石棉纤维可能对细胞施加了氧化应激，从而导致这些畸变。
