乙型流感病毒RNA体外转录的突变分析
Mutational analysis of influenza B virus RNA transcription in vitro.
作者信息
Lee Y S, Seong B L
机构信息
Institute of Biological Sciences, Hanhyo Institutes of Technology, Kyungki-Do, South Korea.
出版信息
J Virol. 1996 Feb;70(2):1232-6. doi: 10.1128/JVI.70.2.1232-1236.1996.
Abstract
The roles of the 3'- and 5'-terminal nucleotides and the panhandle structure of influenza B virus virion RNA were analyzed in vitro by transcription of model RNA templates with influenza B virus RNA polymerase. The results suggest that the stability of the panhandle and breathing of the extreme ends of the panhandle are important factors for efficient transcription. Influenza B virus polymerase appears to be more tolerant of mutations in the panhandle structure than influenza A virus polymerase. This is consistent with the greater degree of heterogeneity observed naturally in the 3'-terminal nucleotides of the virion RNA of influenza B virus than in influenza A virus.
摘要
通过用乙型流感病毒RNA聚合酶转录模型RNA模板，在体外分析了乙型流感病毒病毒粒子RNA的3'和5'末端核苷酸以及锅柄结构的作用。结果表明，锅柄的稳定性和锅柄末端的松动是有效转录的重要因素。与甲型流感病毒聚合酶相比，乙型流感病毒聚合酶似乎对锅柄结构中的突变更具耐受性。这与在乙型流感病毒病毒粒子RNA的3'末端核苷酸中比在甲型流感病毒中自然观察到的更大程度的异质性一致。
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