通过核磁共振光谱研究甲型流感病毒锅柄状RNA的二级结构。
Secondary structure of the panhandle RNA of influenza virus A studied by NMR spectroscopy.
作者信息
Cheong H K, Cheong C, Choi B S
机构信息
Department of Chemistry, Korea Advanced Institute of Science and Technology, Yusung-gu, Taejon.
出版信息
Nucleic Acids Res. 1996 Nov 1;24(21):4197-201. doi: 10.1093/nar/24.21.4197.
Abstract
The double-stranded panhandle structure of the influenza virus RNA is important for replication, transcription and packaging into the virion of the virion RNA. The solution structure of a 34 nt RNA which contains the conserved panhandle sequences has been investigated by one- and two-dimensional NMR spectroscopy. The partially complementary 5'- and 3'-ends of the RNA form a double helical structure which is, on average, close to A-form. The stem contains bulges at nucleotides A10, A12 and C26. In between these bulges, C11 and G25 form a Watson-Crick base pair. The structural features of the panhandle provide a framework for the explanation of mutational analysis and for a better understanding of RNA-polymerase interactions.
摘要
流感病毒RNA的双链锅柄状结构对于病毒粒子RNA的复制、转录以及包装到病毒粒子中都很重要。通过一维和二维核磁共振光谱研究了一段包含保守锅柄序列的34个核苷酸RNA的溶液结构。RNA部分互补的5'端和3'端形成了一个平均接近A-型的双螺旋结构。茎在核苷酸A10、A12和C26处有凸起。在这些凸起之间，C11和G25形成一个沃森-克里克碱基对。锅柄状结构的特征为解释突变分析以及更好地理解RNA聚合酶相互作用提供了一个框架。
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1
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J Gen Virol. 1993 Jul;74 ( Pt 7):1327-33. doi: 10.1099/0022-1317-74-7-1327.
2
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3
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Infect Agents Dis. 1993 Feb;2(1):17-24.
4
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J Virol. 1994 Mar;68(3):1509-15. doi: 10.1128/JVI.68.3.1509-1515.1994.
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EMBO J. 1994 Jul 1;13(13):3158-65. doi: 10.1002/j.1460-2075.1994.tb06614.x.
6
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7
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8
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J Virol. 1994 Feb;68(2):1245-9. doi: 10.1128/JVI.68.2.1245-1249.1994.
9
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Gene. 1980 Feb;8(3):315-28. doi: 10.1016/0378-1119(80)90007-4.
10
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