Cheong H K, Cheong C, Choi B S
Department of Chemistry, Korea Advanced Institute of Science and Technology, Yusung-gu, Taejon.
Nucleic Acids Res. 1996 Nov 1;24(21):4197-201. doi: 10.1093/nar/24.21.4197.
The double-stranded panhandle structure of the influenza virus RNA is important for replication, transcription and packaging into the virion of the virion RNA. The solution structure of a 34 nt RNA which contains the conserved panhandle sequences has been investigated by one- and two-dimensional NMR spectroscopy. The partially complementary 5'- and 3'-ends of the RNA form a double helical structure which is, on average, close to A-form. The stem contains bulges at nucleotides A10, A12 and C26. In between these bulges, C11 and G25 form a Watson-Crick base pair. The structural features of the panhandle provide a framework for the explanation of mutational analysis and for a better understanding of RNA-polymerase interactions.
流感病毒RNA的双链锅柄状结构对于病毒粒子RNA的复制、转录以及包装到病毒粒子中都很重要。通过一维和二维核磁共振光谱研究了一段包含保守锅柄序列的34个核苷酸RNA的溶液结构。RNA部分互补的5'端和3'端形成了一个平均接近A-型的双螺旋结构。茎在核苷酸A10、A12和C26处有凸起。在这些凸起之间，C11和G25形成一个沃森-克里克碱基对。锅柄状结构的特征为解释突变分析以及更好地理解RNA聚合酶相互作用提供了一个框架。
