Nohmi T, Abe S, Dobashi K, Tansho S, Yamaguchi H
Department of Microbiology and Immunology, Teikyo University School of Medicine, Tokyo, Japan.
Microbiol Immunol. 1995;39(6):405-9. doi: 10.1111/j.1348-0421.1995.tb02220.x.
Sex steroid hormones were examined for their effect on mycelial growth of Candida albicans, and the inhibitory activity of casein-induced murine peritoneal neutrophils against mycelial growth of C. albicans was examined in vitro using a crystal violet staining method or a [3H]glucose incorporation method. Four steroid hormones, danazol, estradiol, estriol and testosterone had no effect on mycelial growth of C. albicans, but progesterone appeared to convert the growth form of C. albicans from hyphal to yeast. Danazol (10(-6) M) and progesterone (10(-5) M) suppressed anti-Candida activity of neutrophils of non-treated mice, while testosterone, estradiol, and estriol did not. The anti-Candida activity of neutrophils of estradiol-pretreated mice was clearly suppressed by progesterone even at 10(-6) M which corresponded to its plasma concentration in pregnant women in the third trimester. The physiological significance of this suppressive effect of progesterone was discussed in relation to the vulnerability of pregnant women to vaginal candidiasis.
研究了性类固醇激素对白色念珠菌菌丝体生长的影响，并使用结晶紫染色法或[3H]葡萄糖掺入法在体外检测了酪蛋白诱导的小鼠腹腔中性粒细胞对白色念珠菌菌丝体生长的抑制活性。达那唑、雌二醇、雌三醇和睾酮这四种类固醇激素对白色念珠菌的菌丝体生长没有影响，但孕酮似乎能将白色念珠菌的生长形式从菌丝转变为酵母。达那唑（10(-6) M）和孕酮（10(-5) M）抑制了未处理小鼠中性粒细胞的抗念珠菌活性，而睾酮、雌二醇和雌三醇则没有。即使在10(-6) M（相当于妊娠晚期孕妇血浆浓度）时，孕酮也能明显抑制经雌二醇预处理小鼠中性粒细胞的抗念珠菌活性。讨论了孕酮这种抑制作用的生理意义与孕妇易患阴道念珠菌病的关系。
