Singh S P, Abraham S K, Kesavan P C
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Mutat Res. 1995 Dec;345(3-4):147-53. doi: 10.1016/0165-1218(95)90050-0.
Garlic extract was evaluated in the mouse bone marrow micronucleus test for its possible protective effects against gamma-radiation-induced chromosomal damage. Together with this, biochemical assays were carried out to determine the changes in sulfhydryl content and glutathione S-transferase activities. Three doses of freshly prepared garlic extract (125, 250 and 500 mg/kg b.w.) were orally administered for 5 consecutive days, and the animals were irradiated 2 h after the final feeding. The results of the micronucleus test demonstrated that pre-treatment with garlic extract can lead to significant dose-related reductions in the frequencies of gamma-radiation-induced (2 Gy) micronucleated polychromatic erythrocytes. The anticlastogenic effect of garlic extract was observed against lower radiation doses of 0.5 and 1 Gy, but not 0.25 Gy. Significant increases in the sulfhydryl content and glutathione S-transferase activity were observed after either pre-treatment with garlic extract or irradiation. However, the irradiated garlic-extract pre-treated animals showed a significant reduction in sulfhydryl content and glutathione S-transferase activities.
在小鼠骨髓微核试验中评估了大蒜提取物对γ辐射诱导的染色体损伤的可能保护作用。与此同时，进行了生化分析以确定巯基含量和谷胱甘肽S-转移酶活性的变化。连续5天口服给予三种剂量的新鲜制备的大蒜提取物（125、250和500mg/kg体重），并在最后一次喂食后2小时对动物进行照射。微核试验结果表明，用大蒜提取物预处理可导致γ辐射诱导（2Gy）的微核多色红细胞频率显著剂量相关降低。在较低辐射剂量0.5和1Gy下观察到大蒜提取物的抗断裂效应，但在0.25Gy下未观察到。在用大蒜提取物预处理或照射后，均观察到巯基含量和谷胱甘肽S-转移酶活性显著增加。然而，经大蒜提取物预处理后再照射的动物巯基含量和谷胱甘肽S-转移酶活性显著降低。
