Singh S P, Abraham S K, Kesavan P C
School of Life Sciences, Jawaharlal Nehru Unviersity, New Delhi, India.
Br J Cancer Suppl. 1996 Jul;27:S102-4.
Freshly prepared aqueous extract of garlic was tested in mice for its possible in vivo protective effect against gamma-radiation-induced chromosomal damage. In the same animals, the changes in the sulphydryl content and glutathione S-transferase activity were evaluated. Three doses of garlic extract [125, 250 and 500 mg kg-1 body weight (bw)] were administered orally for five consecutive days and the animals were exposed to 0.25, 0.5, 1.0 and 2.0 Gy gamma-radiation 2 h after the final feeding. The results of the bone marrow micronucleus test revealed that pretreatment with garlic extract was effective in reducing gamma-radiation-induced chromosomal damage. Against 0.25 Gy gamma-radiation, a high dose of 500 mg kg-1 bw garlic extract was required to significantly reduce the chromosomal damage. All the three doses of garlic extract were effective in exerting a protective effect against 0.5, 1.0 and 2.0 Gy gamma-radiation. However a dose-related effect was observed only against 2.0 Gy. The sulphydryl content and glutathione S-transferase activity registered a significant increase after either pretreatment with garlic with extract or irradiation. In the garlic extract pretreated irradiated animals, a significant reduction was observed in the sulphydryl content and glutathione S-transferase activity.
对新鲜制备的大蒜水提取物进行了小鼠实验，以研究其对γ射线诱导的染色体损伤可能的体内保护作用。在相同的动物中，评估了巯基含量和谷胱甘肽S-转移酶活性的变化。连续五天口服给予三种剂量的大蒜提取物[125、250和500毫克/千克体重（bw）]，在最后一次喂食后2小时，将动物暴露于0.25、0.5、1.0和2.0 Gy的γ射线下。骨髓微核试验结果表明，用大蒜提取物预处理可有效减少γ射线诱导的染色体损伤。对于0.25 Gy的γ射线，需要500毫克/千克体重的高剂量大蒜提取物才能显著减少染色体损伤。所有三种剂量的大蒜提取物对0.5、1.0和2.0 Gy的γ射线均有保护作用。然而，仅在2.0 Gy时观察到剂量相关效应。在用大蒜提取物预处理或照射后，巯基含量和谷胱甘肽S-转移酶活性显著增加。在用大蒜提取物预处理的受照射动物中，巯基含量和谷胱甘肽S-转移酶活性显著降低。
