Chowen J A, Busiguina S, García-Segura L M
Instituto Cajal, C.S.I.C., Madrid, Spain.
Neuroscience. 1995 Nov;69(2):519-32. doi: 10.1016/0306-4522(95)00250-m.
By using the techniques of in situ hybridization histochemistry and immunocytochemistry, we have found that both glial fibrillary acidic protein messenger RNA levels and glial fibrillary acidic protein immunoreactive surface density in the arcuate nucleus and median eminence are modulated by both the neonatal and adult sex steroid environments. No effect was seen on the number of immunoreactive glia. Intact adult males had significantly higher glial fibrillary acidic protein messenger RNA levels and glial fibrillary acidic protein immunoreactive surface density than females. Both adult and neonatal castration of male animals significantly reduced glial fibrillary acidic protein messenger RNA levels and glial fibrillary acidic protein immunoreactive surface density. Neonatal and adult testosterone treatment increased both of these parameters in both sexes; however, there was no additive effect of the steroid treatments. Glial cells are involved in the proliferation, survival, migration and maturation of neurons, as well as in the modulation of synaptic connectivity, and therefore it follows that hormonal modulation of glia may mediate some of the known steroid effects on neurons. The data reported here show that astroglia are significantly influenced by both the neonatal and adult sex steroid environments and suggest that some of the steroid effects on neurons during both of these developmental periods may be mediated, at least in part, through modulation of glial cells.
通过使用原位杂交组织化学和免疫细胞化学技术，我们发现弓状核和正中隆起中胶质纤维酸性蛋白信使核糖核酸水平以及胶质纤维酸性蛋白免疫反应性表面密度均受到新生期和成年期性类固醇环境的调节。未观察到对免疫反应性胶质细胞数量有影响。完整的成年雄性动物的胶质纤维酸性蛋白信使核糖核酸水平和胶质纤维酸性蛋白免疫反应性表面密度显著高于雌性。对雄性动物进行成年期和新生期去势均显著降低了胶质纤维酸性蛋白信使核糖核酸水平和胶质纤维酸性蛋白免疫反应性表面密度。新生期和成年期睾酮处理均增加了两性的这两个参数；然而，类固醇处理没有叠加效应。胶质细胞参与神经元的增殖、存活、迁移和成熟，以及突触连接的调节，因此，对胶质细胞的激素调节可能介导了一些已知的类固醇对神经元的作用。此处报告的数据表明，星形胶质细胞受到新生期和成年期性类固醇环境的显著影响，并提示在这两个发育时期，类固醇对神经元的一些作用可能至少部分是通过对胶质细胞的调节介导的。
