Scali C, Vannucchi M G, Pepeu G, Casamenti F
Department of Preclinical and Clinical Pharmacology, University of Florence, Italy.
Neurosci Lett. 1995 Sep 15;197(3):171-4. doi: 10.1016/0304-3940(95)11914-i.
The effect of intraperitoneal administration of scopolamine (1 mg/kg) on acetylcholine (ACh) release in vivo in 3- and 24-month-old freely behaving rats was investigated in the cerebral cortex, hippocampus and striatum by means of transverse microdialysis. In the parietal cortex, the increase in ACh release after scopolamine administration was significantly greater in the old than in the young rats, reaching a maximum increase of about 600 and 300% in the old and young animals, respectively. In the hippocampus, scopolamine caused a larger increase in ACh release in the young (+900%) than in the old rats (+600%). In the striatum of aged rats, a 40% increase occurred only at 40 min after scopolamine administration while in the striatum of young animals the increase lasted for at least 2 h, reaching a maximum of about 100%. These findings demonstrate that the modulation of ACh release in vivo is affected in a different manner in the cerebral cortex than in the hippocampus and striatum by aging.
通过横向微透析法，研究了腹腔注射东莨菪碱（1毫克/千克）对3月龄和24月龄自由活动大鼠大脑皮层、海马体和纹状体内乙酰胆碱（ACh）释放的影响。在顶叶皮层，东莨菪碱给药后，老年大鼠ACh释放的增加显著大于年轻大鼠，老年和年轻动物的最大增加量分别约为600%和300%。在海马体中，东莨菪碱引起年轻大鼠（增加900%）ACh释放的增加幅度大于老年大鼠（增加600%）。在老年大鼠的纹状体中，仅在东莨菪碱给药后40分钟出现了40%的增加，而在年轻动物的纹状体中，增加持续至少2小时，最大增加约为100%。这些发现表明，衰老对大脑皮层中ACh释放的体内调节影响方式与海马体和纹状体不同。
