Khan W I, Abe T, Ishikawa N, Nawa Y, Yoshimura K
Department of Parasitology, Akita University School of Medicine, Japan.
Parasite Immunol. 1995 Sep;17(9):485-91. doi: 10.1111/j.1365-3024.1995.tb00919.x.
Mechanism of spontaneous cure was studied in mice infected with mouse-nonadaptive Nippostrongylus brasiliensis. Adult BALB/c mice were cured spontaneously of infection with this strain of N. brasiliensis by Day 7 post-infection. Expulsion of intestinal worms was delayed dose-dependently by a treatment with anti-CD4 antibody. However, the treatment had no significant effect on larval recovery from the lungs. Treatment of mice with anti-IL-5 antibody suppressed intestinal tissue eosinophilia induced by the infection, but did not affect intestinal worm recovery. Antigen specific IgE antibody was not detected in the sera obtained from Days 5 to 15. Therefore, IL-5 and specific IgE antibody are probably not important in the spontaneous cure. Treatment of mice with anti-CD4 antibody had no significant effect on number of intestinal goblet cells or on expression of terminal sugars of goblet cell mucins. However, histological and quantitative analyses revealed that significantly less intestinal mucus was released in anti-CD4 antibody treated mice than in control mice. These results suggest that CD4+ lymphocytes control the amount of intestinal mucus and consequently the reduced mucus interferes with the spontaneous cure. Quantity of mucus released in the intestinal lumen may have an essential role in the spontaneous cure of N. brasiliensis-infection of mice.
研究了感染非适应性巴西日圆线虫的小鼠的自愈机制。成年BALB/c小鼠在感染后第7天可自发清除该株巴西日圆线虫感染。用抗CD4抗体处理可剂量依赖性地延迟肠道蠕虫的排出。然而，该处理对从肺中回收幼虫没有显著影响。用抗IL-5抗体处理小鼠可抑制感染诱导的肠道组织嗜酸性粒细胞增多，但不影响肠道蠕虫的回收。在感染后第5天至第15天获得的血清中未检测到抗原特异性IgE抗体。因此，IL-5和特异性IgE抗体可能在自愈过程中并不重要。用抗CD4抗体处理小鼠对肠道杯状细胞数量或杯状细胞粘蛋白末端糖的表达没有显著影响。然而，组织学和定量分析显示，抗CD4抗体处理的小鼠释放的肠道黏液明显少于对照小鼠。这些结果表明，CD4+淋巴细胞控制肠道黏液的量，因此减少的黏液会干扰自愈。肠腔内释放的黏液量可能在小鼠巴西日圆线虫感染的自愈中起重要作用。
