Camacho Villarreal N G, Ventura-Juárez J, Castañeda-Ibarra F J, Ruiz-Maya L, García-Latorre E, Campos-Rodríguez R
Departamento de Bioquímica, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón, México, D.F., Mexico.
Rev Latinoam Microbiol. 1995 Apr-Jun;37(2):153-60.
The mechanisms of immune response generation and regulation at the intestinal level are not well known, mainly due to the lack of suitable and reproducible methods to measure local immune responses. The Cunningham direct and indirect hemolytic plaque assay for the quantification of antibody producing cells against Salmonella in Peyer's patches of mice orally infected with Salmonella typhimurium was used. After infection IgM and IgG producing cells were determined on days 6, 9, 12 and 19. Specific antibody producing cells appeared after bacterial invasion of Peyer's patches, nine days after infection. At this time, there were more antibody producing cells in the distal part of the intestine, which correlated with a higher infection of these Peyer's patches as detected by bacterial culture. After day nine, the number of plaque forming cells was similar in both parts of the intestine. The peak of response was on day 12 and diminished on day 19. The number of IgM and IgG producing cells was similar in all days analyzed. Histological analysis of Peyer's patches of infected mice showed inflammation with disorganization and tumefaction.
肠道水平免疫反应产生和调节的机制尚不清楚，主要是由于缺乏合适且可重复的方法来测量局部免疫反应。使用了坎宁安直接和间接溶血空斑试验来定量口服感染鼠伤寒沙门氏菌的小鼠派尔集合淋巴结中针对沙门氏菌的抗体产生细胞。感染后，在第6、9、12和19天测定产生IgM和IgG的细胞。在细菌侵入派尔集合淋巴结后，即感染后九天，出现了特异性抗体产生细胞。此时，肠道远端有更多的抗体产生细胞，这与通过细菌培养检测到的这些派尔集合淋巴结更高的感染率相关。在第九天后，肠道两部分的空斑形成细胞数量相似。反应高峰在第12天，第19天有所下降。在所有分析的日子里，产生IgM和IgG的细胞数量相似。对感染小鼠派尔集合淋巴结的组织学分析显示有炎症，伴有结构紊乱和肿胀。
