大鼠派尔集合淋巴结手术切除对肠道抗原全身抗体反应的影响。
The effects of surgical removal of Peyer's patches in rat on systemic antibody responses to intestinal antigen.
作者信息
Heatley E V, Stark J M, Horsewood P, Bandouvas E, Cole F, Bienenstock J
出版信息
Immunology. 1981 Nov;44(3):543-8.
A method is described for the in vivo surgical removal of all Peyer's patches from the small intestine and caecum of the rat. Over two postoperative months, this procedure had no apparent morphological effects on the small intestine or intraepithelial lymphocyte numbers, nor were the numbers of IgA producing cells in the small intestine or serum immunoglobulin levels permanently influenced. However, circulating specific antibody to human serum albumin was significantly elevated in animals without Peyer's patches 3 weeks after surgery, in comparison with a sham operated group of animals. Subsequent intestinal immunization in animals without Peyer's patches with a lipid-conjugated human serum albumin resulted in a diminished primary but a comparatively normal secondary systemic antibody response to this antigen.
本文描述了一种在大鼠体内通过手术切除小肠和盲肠所有派尔集合淋巴结的方法。术后两个月内，该手术对小肠或上皮内淋巴细胞数量没有明显的形态学影响，小肠中产生IgA的细胞数量或血清免疫球蛋白水平也未受到永久性影响。然而，与假手术组动物相比，无派尔集合淋巴结的动物在手术后3周，针对人血清白蛋白的循环特异性抗体显著升高。随后，对无派尔集合淋巴结的动物用脂质偶联的人血清白蛋白进行肠道免疫，导致对该抗原的初次全身抗体反应减弱，但二次全身抗体反应相对正常。
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